Study on the therapeutic effect of compound Yijing decoction on cyclophosphamide-induced premature ovarian failure in mice
Objective To explore the therapeutic effect of traditional Chinese medicine compound Yijing decoction on cyclophosphamide(CTX)-induced premature ovarian failure(POF)in mice.Methods The POF mouse model was established by intraperitoneal injection of CTX,and was randomly divided into the model group,positive control group,low and high doses of Yijing decoction groups,and blank group,with 6 mice in each group.The drug was administered by gavage for 30 d.The estrous cycle,body weight,ovarian and uterine indices,and changes in ovarian and uterine tissues of mice were measured.The concentration of serum E2,FSH,LH,AMH and PROG were detected with ELISA.The levels of ovarian PI3K,AKT,and FOXO3A protein were detected by immunohistochemistry(IHC).Results After intervention with Yijing decoction in mice with POF,the estrus cycle tended to be normal.The body weight,ovarian index,and uterine index significantly increased in the high-dose group mice(P<0.05).The ovarian volume and the number of primary and secondary follicles were increased,and the phenomenon of interstitial and fibrosis was improved.The endometrium was significantly thickened,and the arrangement of endometrial glandular epithelial cells was restored.The levels of serum E2,AMH and PROG were increased in both the positive control group and the high-dose Yijing decoction group(P<0.05,P<0.01),while levels of FSH and LH were significantly decreased(P<0.01).Meantime,the expression of PI3K,AKT and FOXO3A proteins was significantly reduced in both two groups(P<0.01).Conclusion Yijing decoction can effectively improve the motility cycle and restore the function of ovary and uterus,increase the levels of serum E2,AMH,PROG,and decrease the expression of FSH and LH,which may be related to downregulation of the expression of PI3K,AKT and FOXO3A proteins.
万方数据
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