Objective The pumpkin seed standard decoction was prepared,and its quality evaluation method was established to provide reference for the formulation of pumpkin seed formula granules quality standard.Methods According to the traditional decocting method,15 batches of pumpkin seed standard decoction were prepared,and the range of the paste yield was determined.The quality standard of the standard decoction was established,including the qualitative identification with alanine by TLC,the establishment of characteristic spectra and mass fraction determination method by HPLC,and the calculation of their transfer rate.Results The extraction rate of 15 batches of pumpkin seed standard decoction ranged from 10.4%to 18.3%,with an average of 15.1%.TLC spots were clear,the separation degree was good,and the specific shift value was appropriate.The mass fraction of phenylalanine ranged from 0.56 to 1.99 mg/g with an average value of 1.43 mg/g,and the transfer rate ranged from 42.7%to 86.1%with an average value of 69.7%.The established eigenmaps showed 13 characteristic peaks,and the similarity was all greater than 0.9.Conclusion The established method of quality standard of pumpkin seed standard decoction is comprehensive and accurate,which can provide reference for the formulation of pumpkin seed formula granules.
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