HPLC法测定骨伤跌打康复凝胶贴膏剂中7种成分含量

扫码查看

原文链接

万方数据
维普

中文摘要：目的建立测定骨伤跌打康复凝胶贴膏剂中7种主要成分质量分数的HPLC法.方法色谱柱为Agilent ZORBA×SB-C18 S tableBond Analytical柱(4.6 mm×250 mm,5 µm);测定波长为290 nm,流动相为乙腈-0.1%甲酸水梯度洗脱;流速为1.0 mL/min;柱温为 35℃;进样量为10 µL.结果骨伤跌打康复凝胶贴膏剂中的原儿茶酸、咖啡酸、阿魏酸、盐酸小檗碱、大黄酸、大黄素、大黄酚7种主要有效成分在浓度线性范围内与色谱峰面积的标准曲线线性关系良好;原儿茶酸、咖啡酸、阿魏酸、盐酸小檗碱、大黄酸、大黄素、大黄酚的平均回收率分别为93.79%、95.86%、96.12%、95.31%、96.58%、107.43%、97.12%,7种成分的平均回收率均在93.79%～107.43%范围内.结论建立的方法适合作为骨伤跌打康复凝胶贴膏剂质量控制的方法.

外文标题：Determination of seven components in Gushang Dieda Kangfu Cataplasm by HPLC

外文摘要：Objective To establish an HPLC method for the determination of the seven main components of Gushang Dieda Kangfu Cataplasm.Methods The chromatographic column was Agilent ZORBA×SB-C18 S tableBond Analytical column,(4.6 mm×250 mm,5 µm),the detection wavelength was 290 nm,and the mobile phase was acetonitrile-0.1%formic acid water by gradient elution at a flow rate 1.0 mL/min.The column temperature was 35℃,the injection volume was 10 µL.Results There were good linear relationships between the standard curves of the chromatographic peak area and the concentration linear range of protocatechuic acid,caffeic acid,ferulic acid,berberine hydrochloride,rhein,emodin and chrysophanol in the Gushang Dieda Kangfu Cataplasm.The average recoveries were 93.79%,95.86%,96.12%,95.31%,96.58%,107.43%,97.12%,and they were between 93.79%-107.43%.Conclusion This established method is suitable for the quality control of the Gushang Dieda Kangfu Cataplasm.

外文关键词：

Gushang Dieda Kangfu CataplasmHPLCprotocatechuic acidcaffeic acidferulic acidberberine hydrochlorideRheinrhubarbchrysophanol

作者：

吴谋、莫楚铭、蓬展鹏、刘淑芳、陈继英

展开 >

作者单位：

东莞市中医院,广东东莞 523000

东莞市中药制剂重点实验室,广东东莞 523000

关键词：

骨伤跌打康复凝胶贴膏剂高效液相色谱法原儿茶酸咖啡酸阿魏酸盐酸小檗碱大黄酸大黄素大黄酚

基金：

东莞市社会发展科技项目重点项目

项目编号：

20211800904392

出版年：

2024

DOI：

10.16809/j.cnki.2096-3653.2023091203

广东药科大学学报

广东药学院

广东药科大学学报

CSTPCD

影响因子：0.698

ISSN：1006-8783

年,卷(期)：2024.40(1)

参考文献量10