Preliminary study on the transcutaneous permeability and anti-psoriasis effect of clobetasolpropioate fatty acid vesicles
Objective To investigate the in vitro permeability of clobetasolpropioatefatty acid vesicles(CP-FAV)and its efficacy in psoriasis model mice.Methods The in vitro transdermal study was conducted by Franz diffusion pool,and the effect of fatty acid vesicles on skin structure was investigated by differential scanning thermal analysis(DSC)and Fourier transform infrared spectroscopy(FTIR).Mouse psoriasis model was constructed to evaluate skin lesions by psoriasis area and severity index(PASI),and hematoxylin-eosin(HE)staining was used to analyze the histopathological changes of the lesions.Results The in vitro transdermal assay showed that CP-FAV could effectively improve the transdermal absorption capacity of CP,and its diffusion curve in vitro fitted the first-order kinetic equation,which could effectively and continuously release CP through skin.FTIR results showed that fatty acid vesicles could change lipid ordering,and DSC results showed that fatty acid vesicles could improve cuticle lipid mobility and promote the osmotic transfer of clobetasol propionate in skin cuticle.The results of pharmacodynamic experiments showed that the CP-FAV group could significantly reduce PASI score(P<0.05)and improve the skin damage of psoriasis-like mice compared with the model group;HE staining showed that the thickness of the epidermal layer in mouse skin lesions and the inflammatory cell infiltration in the dermis of mice were significantly reduced.Conclusion Fatty acid vesicles could increase the permeability of CP for local application in the skin,and reduce the skin barrier to drugs.It is a new local delivery system for the treatment of psoriasis.
万方数据
维普
