Establishment and comparison of acute and chronic ulcerative colitis mice models
Objective To investigate the physiological and pathological changes of mice models of chronic ulcerative colitis(UC)induced by DSS and acute ulcerative colitis induced by OXZ,and to provide evidence for the selection of animal model for the development of therapeutic drugs for ulcerative colitis.Methods Chronic ulcerative colitis was induced in C57BL/6N mice by alternating drinking water with or without 1%DSS for 7 consecutive weeks.Acute ulcerative colitis was induced in Balb/c mice by abdominal sensitization with OXZ combined with ethanol enema.After the establishment of the model,the modeling effect was evaluated through disease activity index(DAI)score,organ index,hematological examination,colon tissue HE staining,and ELISA detection of TNF-α,INF-γ and IL-10 levels,and a comparative study was conducted between the two models.Results In the DSS and OXZ induced mouse colitis model,the model group mice showed clinical symptoms of UC such as decreased body weight,loose stools,and bloody stools.The DAI score was significantly increased(P<0.01).Compared with the control group,the thymus index of the model mice was significantly reduced,the spleen index was significantly increased,and the colon length was significantly shortened(P<0.01).The results of hematological examination and related inflammatory factor detection showed that the model group mice experienced inflammatory reactions.The histopathological examination revealed pathological features such as ulceration and inflammatory cell infiltration in the colon of the model group mice.Conclusion Both methods can induce ulcerative colitis in mice,but the physiological state of mice and the pathological changes of colon are different.In the actual experiment,appropriate model should be selected according to the research purpose and the characteristics of drug therapy.
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