广东药科大学学报2024，Vol.40Issue(2) ：23-31.DOI:10.16809/j.cnki.2096-3653.2024020503

基于UPLC特征图谱和含量测定的蜜远志标准汤剂质量评价研究

Study on quality evaluation of Polygalae Radix Praeparata Cum Melle standard decoction based on UPLC characteristic chromatograms and content determination

严慕贤 蓝义琨 谭有珍 梁盛 周雅亮 吴颖欣
广东药科大学学报2024，Vol.40Issue(2) ：23-31.DOI:10.16809/j.cnki.2096-3653.2024020503
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  • 维普
  • 万方数据

基于UPLC特征图谱和含量测定的蜜远志标准汤剂质量评价研究

Study on quality evaluation of Polygalae Radix Praeparata Cum Melle standard decoction based on UPLC characteristic chromatograms and content determination

严慕贤 1蓝义琨 1谭有珍 1梁盛 1周雅亮 1吴颖欣1
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作者信息

  • 1. 佛山市中医院,广东佛山 528000
  • 折叠

摘要

目的 建立蜜远志标准汤剂特征图谱以及3种成分质量分数同时测定的UPLC方法,对不同来源蜜远志饮片制成的标准汤剂质量进行评价.方法 采用UPLC法建立蜜远志标准汤剂的特征图谱以及细叶远志皂苷、远志(山)酮Ⅲ和3,6′-二芥子酰基蔗糖质量分数测定的方法,并对16批标准汤剂进行测定,采用高分辨质谱对共有峰进行了指认,采用聚类分析及偏最小二乘法分析(PLS-DA)对共有峰进行了分析.结果 蜜远志标准汤剂特征图谱标定了9个共有峰,通过高分辨质谱分别指认为:西伯利亚远志糖A5、西伯利亚远志糖A6、远志(山)酮Ⅲ、3,6′-二芥子酰基蔗糖、黄花远志素A、细叶远志皂苷A、远志寡精H、远志寡糖A、1,2,3,7-四甲氧基呫吨酮,相似度结果显示16批样品的相似度均大于0.900.聚类分析及PLS-DA显示不同来源之间的样品可能存在质量差异.结论 本研究建立的蜜远志标准汤剂特征图谱和质量分数测定方法简便、快速、准确,可为蜜远志相关制剂的质量评价提供参考.

Abstract

Objective To establish the UPLC method of Characteristic chromatograms and three components content determination of Polygalae Radix Praeparata Cum Melle standard decoction in order to evaluate the quality of decoction made by Polygalae Radix Praeparata Cum Melle docoction pieces from different origins.Methods The UPLC was used to establish the Characteristic chromatograms and tenuifolin,polygalaxanthone Ⅲ and 3,6′-disinapoyl sucrose content determination method of Polygalae Radix Praeparata Cum Melle standard decoction,and 16 batches of standard decoctions were determined.The high resolution mass spectrometer was used to identify the common peaks and the cluster analysis and partial least squares discriminant analysis(PLS-DA)were used to analyze the common peaks.Results Nine common peaks were determined in the Characteristic chromatograms of Polygalae Radix Praeparata Cum Melle standard decoction while the peaks were identified as sibiricose A5,sibiricose A6,polygalaxanthoneⅢ,3,6′-disinapoyl sucrose,arillanin A,tenuifolin A,tenuifoliose H,tenuifoliose A and 1,2,3,7-tetramethoxyxanthone by high resolution mass spectrometer.The similarities of the 16 batches samples were all greater than 0.900.Cluster analysis and PLS-DA showed there might be differences among the samples from different origins.Conclusion The established Characteristic chromatogram and content determination method of Polygalae Radix Praeparata Cum Melle standard decoction is simple,quick and accurate,which can provide a reference for the quality evaluation of Polygalae Radix Praeparata Cum Melle relative preparations.

关键词

蜜远志/标准汤剂/特征图谱/含量测定/质量评价

Key words

Polygalae Radix Praeparata Cum Melle/standard decoction/characteristic chromatogram/content determination/quality evaluation

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基金项目

佛山市卫生计生局医学科研项目(20190095)

出版年

2024
广东药科大学学报
广东药学院

广东药科大学学报

CSTPCD
影响因子：0.698
ISSN：1006-8783
参考文献量23
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