Study on UPLC fingerprints and multi-component content determination of Jiangtang Xiaoke Granules
Objective To establish UPLC fingerprints of Jiangtang Xiaoke Granules and a method for the content determination of 5 index components.Methods The chromatographic column Waters UPLC T3(2.1 mm×150 mm,1.6 μm)was selected with 0.1%phospho-methanol as mobile phase by gradient elution,and the detection wavelength was 254 nm.The flow rate was 0.2 mL/min.The column temperature was 30℃,and the injection volume was 1 μL.The fingerprints of 15 batches of samples were established by UPLC method.SPSS and SIMCA software were used for statistical analysis,and the content of chemical components was determined.Results A total of 21 common peaks were identified.The chemometrics results divided the samples into 2 categories and screened out 5 different components:Berberine hydrochloride,Palmatine hydrochloride,Jatrorrhizine Hydrochloride,Coptisine and Epiberberine.Berberine hydrochloride had a good linear relationship between 20.550 0-205.500 0 μg(r=0.999 8)and the average recovery rate was 100.68%.Palmatine hydrochloride had a good linear relationship between 7.690 0-57.654 0 μg(r=0.999 4)and the average recovery rate was 99.99%.Jatrorrhizine hydrochloride had a good linear relationship between 7.920 0-47.520 0 μg(r=0.999 8)and the average recovery rate was 98.81%.Coptisine had a good linear relationship between 8.915 0-66.660 0 μg(r=0.999 8)and the average recovery rate was 99.01%.Epiberberine had a good linear relationship between 8.108 0-60.810 0 μg(r=0.999 8)and the average recovery rate was 98.87%.Conclusion The established fingerprint of Jiangtang Xiaoke Granules and its content determination method are stable and reliable,which can provide scientific reference material for the quality specification of this preparation.
万方数据
维普
