首页|星点设计-效应面法优化白及多糖pH-离子敏感原位凝胶的处方

星点设计-效应面法优化白及多糖pH-离子敏感原位凝胶的处方

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目的 优化白及多糖pH-离子敏感原位凝胶的处方.方法 以去乙酰结冷胶用量、海藻酸钠用量、氯化钙用量、枸橼酸钠与氯化钙的质量比为考察变量,采用单因素实验初步确定各变量的范围.以25℃凝胶前体溶液黏度和37℃胶凝黏度为考察指标,采用星点设计-效应面法优选白及多糖pH-离子敏感原位凝胶的处方工艺,并进行预测分析.结果 白及多糖pH敏感原位凝胶的最优处方为:去乙酰结冷胶用量为0.50%,海藻酸钠用量为1.10%,氯化钙用量为0.07%,枸橼酸钠与氯化钙的质量比为4∶1.该处方条件下,25℃前体溶液黏度为(704.00±3.00)mPa·s,37℃胶凝黏度为(12 544.70±47.33)mPa·s.结论 白及多糖pH-离子敏感原位凝胶稳定性好,前体溶液在25℃流动性良好,凝胶强度良好,可为基于白及多糖原位凝胶的后续研究提供实验基础.
Optimization of the formulation of pH-ionic-sensitive in situ gel of Bletilla striata polysaccharide by central composite design-response surface methodology
Objective To optimize the formulation of pH-ionic-sensitive in situ gel of Bletilla striata polysaccharide.Method The contents of deacetylated gellan gum,sodium alginate,calcium chloride,and the ratio of sodium citrate to calcium chloride were chosen as the variables,the preliminary range of each parameter was determined by the single-factor experiments.The viscosity of the precursor solution at 25℃and the gelation viscosity at 37℃were taken as the evaluation indicators,the central composite design response surface methodology was used to optimize the preparation process and carry out the predictive analysis.Results The optimal preparation conditions for the pH-ionic-sensitive in situ gel of Bletilla striata polysaccharide were as follows:deacetylated gellan gum content of 0.50%,sodium alginate content of 1.10%,calcium chloride content of 0.07%,and a ratio of sodium citrate to calcium chloride of 4∶1.Under these prescription conditions,the viscosity of the precursor solution at 25℃was(704.00±3.00)mPa·s,and the gelation viscosity at 37℃was(12 544.70±47.33)mPa·s.Conclusion The pH-ionic-sensitive in situ gel of Bletilla striata polysaccharide has good stability and flowability of precursor solution at 25℃,and the gel has considerable strength,which provides an experimental basis for further research on in situ gel of Bletilla striata polysaccharide.

Central composite design response surface methodologyBletilla striata polysaccharidepH sensitive in situ geldetermination of viscosity

张忠、范依霖、薛宝玉、蒋琳、仲瑞雪、涂禾

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四川省骨科医院,四川成都 610041

西南交通大学生命科学与工程学院,四川成都 610031

星点设计-效应面法 白及多糖 pH敏感原位凝胶 黏度测定

四川省涂禾名中医工作室建设项目

川中医药办函[2022]36号

2024

广东药科大学学报
广东药学院

广东药科大学学报

CSTPCD
影响因子:0.698
ISSN:1006-8783
年,卷(期):2024.40(2)
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