Analysis of Monocrotaline-hemoglobin adducts in blood using molecularly imprinted polymers
Objective To establish a rapid analytical method for in vivo binding of monocrotaline and hemoglobin based on molecular imprinting technology.Methods The prepared monocrotaline hemoglobin adduct(Mct@BHb)was used as a template molecule to prepare molecularly imprinted polymers(Mct@BHb@MIPs)by using surface imprinting polymerization method,and then the Mct@BHb@MIPs were used as an extraction medium to adsorp toxic markers in rat blood.Results The results showed that the polymer layer was successfully grafted onto the surface of MWCNs,and the polymer exhibited good thermal stability after characterization.The Mct@BHb@MIPs were used as a solid-phase extraction medium to analyze the blood of model rats,and the results showed that the polymer hemoglobin adducts had good adsorption capacity(adsorption amount reached 90.86 mg/mg).The analysis results were consistent with liver pathological detection.Conclusion The polymer prepared by this research can quickly analyze the content of hemoglobin conjugates in vivo,which provides new research ideas for non-invasive diagnosis of hepatic small vein occlusion,combined with pathological results.
万方数据
维普
