Effect of etomidate on myocardial injury in rats with acute myocardial infarction by regulating the AMPK/NLRP3 signaling pathway
Objective To investigate the effect of etomidate on myocardial injury in acute myocardial infarction(AMI)rats by regulating the adenosine monophosphate activated protein kinase(AMPK)/nucleotide binding oligomeric domain-like receptor protein 3(NLRP3)signaling pathway.Methods The AMI model was established in SD rats by ligating the anterior descending branch of the left coronary artery,and the SD rats were randomly grouped into model group,etomidate group,Dorsomorphin group,and etomidate+Dorsomorphin group,with 12 rats in each group,another 12 rats were selected as the sham operation group without ligation after thoracotomy.After grouping and treating,the cardiac fraction,the pathological damage and fibrosis in myocardial tissue,the levels of serum lactate dehydrogenase(LDH),creatine phosphokinase(CPK),IL-1β,IL-6,superoxide dismutase(SOD)and malondialdehyde(MDA),the levels of IL-1β,IL-6,SOD and MDA in myocardial tissue,the expression of AMPK/NLRP3 pathway related proteins in myocardial tissue of rats were measured.Results Compared with the sham surgery group,the myocardial tissue of rats in the model group showed severe pathological damage.The left ventricular end diastolic diameter(LVDD),left ventricular end systolic diameter(LVDS),myocardial collagen volume fraction(CVF),levels of serum LDH,CPK,IL-1β and IL-6,levels of IL-1β,IL-6 and MDA in myocardial tissue,and protein expression of NLRP3 in myocardial tissue were obviously increased(P<0.05).The EF,levels of SOD in serum and myocardial tissue,and p-AMPK/AMPK in myocardial tissue were obviously reduced(P<0.05).Compared with the model group,the pathological damage of myocardial tissue in the etomidate group was reduced.The LVDD,LVDS,myocardial CVF,levels of serum LDH,CPK,IL-1β and IL-6,levels of IL-1β,IL-6 and MDA in myocardial tissue,and protein expression of NLRP3 in myocardial tissue were obviously reduced(P<0.05).The EF,levels of SOD in serum and myocardial tissue,and p-AMPK/AMPK in myocardial tissue were obviously increased(P<0.05).The changes of various indicators in the Dorsomorphin group rats were opposite to that of the etomidate group,and Dorsomorphin weakened the effect of etomidate on various indicators of the model group rats.Conclusion Etomidate can inhibit inflammation and oxidative stress response after AMI by promoting AMPK/NLRP3 signaling,thereby reducing myocardial tissue damage and fibrosis in AMI rats and improving their cardiac function.
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