Effect of pachymoic acid on osteoporosis in ovariectomized mice based on STING/NF-κB signaling pathway
Objective To evaluate the effect of pachymoic acid on osteoporosis in ovariectomized mice by regulating STINGNF-κB signaling pathway.Methods Forty SPF female C57BL/6 mice were divided into sham operation group(Sham),model group(OVX),17β-estradiol group(E2,10µg/kg)and pachymoic acid group(FL,5 mg/kg),and the osteoporosis model of mice was established by bilateral ovariectomy.After 8 weeks,the levels of serum alkaline phosphatase(ALP)and tartrate-resistant acid phosphatase(StrACP)were detected,the morphology of distal femur and the dynamic changes of tibia(cortical bone)bone formation were observed,and the biomechanics of the right femur and the expression levels of key proteins in STING/NF-κB pathway were determined.Results Compared with OVX group,the levels of serum StrACP and ALP in E2 group and FL group were significantly lowered(P<0.01),the area percentage of trabecular bone in distal femur was increased(P<0.01),and the double-fluorescence distance between cortical bones was decreased(P<0.01).The maximum load and fracture load of femur were significantly elevated(P<0.01),and the expression levels of STING and p-NF-κB p65/NF-κB p65 protein in femur were significantly increased(P<0.01).Conclusion Pachymoic acid can inhibit bone resorption and promote bone formation by regulating STING/NF-κB signaling pathway,thereby effectively reducing bone loss in ovariectomized mice.
万方数据
维普
