摘要
目的 研究复方五指毛桃膏对肝郁脾虚型慢性疲劳综合征小鼠的改善作用.方法 构建昆明小鼠肝郁脾虚型慢性疲劳综合征(CFS)病理模型,随机分为空白组、模型组、诺迪康胶囊阳性对照组(145.6 mg/kg)和复方五指毛桃膏高、中、低剂量组(5.2 g/kg、2.6 g/kg、1.3 g/kg).连续灌胃治疗28 d,观测小鼠悬尾实验及旷场实验的活动状态,检测抗氧化应激能力、免疫器官系数和肝糖原、超氧化物歧化酶(SOD)、白细胞介素-2(IL-2)、干扰素-γ(IFN-γ)、5-羟色胺(5-HT)、多巴胺(DA)水平.结果 与模型组相比,复方五指毛桃膏高、中、低剂量组悬尾不动时间缩短(P<0.01)、旷场活动明显增加(P<0.01);在极端环境中生存时间延长(P<0.01);胸腺、脾脏系数均有提高(P<0.01);肝糖原、SOD、IL-2、IFN-γ、5-HT含量升高(P<0.01),DA含量降低(P<0.01).结论 复方五指毛桃膏可增强小鼠抗氧化应激能力,缓解小鼠慢性疲劳和不良情绪,增强机体免疫力,对CFS有较好的疗效.
Abstract
Objective To study the improvement effect of Compound Ficus Hirta Vahl(CFHV)on chronic fatigue syndrome(CFS)in mice.Methods Kunming mice were used to construct a pathological model of chronic fatigue syndrome and randomly divided into the blank group,the model group,the positive control group(145.6 mg/kg),and high,medium,and low doses of CFHV groups(5.2 g/kg,2.6 g/kg,1.3 g/kg).After 28 days of continuous gavage treatment,we observed the behavioral activity of mice in tail suspension and open-field experiments,detected antioxidant stress capacity,immune organ index,and the levels of liver glycogen,super oxide dismutase(SOD),interleukin-2(IL-2),interferon-γ(IFN-γ),5-hydroxy tryptamine(5-HT),and dopamine(DA).Results Compared with the model group,the high,medium,and low doses of CFHV groups showed a shorter tail suspension time,a significant increase in open-field activity,and prolonged survival time in extreme environments(P<0.01).The coefficients of thymus and spleen both were boosted,the levels of liver glycogen,SOD,IL-2,IFN-γ,and 5-HT were increased,but the DA content was decreased(P<0.01).Conclusion CFHV can enhance the antioxidant stress capacity of mice,alleviate chronic fatigue and adverse emotions in mice,enhance the body'immunity,which displays a good therapeutic effect on CFS.
基金项目
广东省中医药局项目(2019401220231249)
维普
万方数据