Effect of Guilu Bushen Wan on airway remodeling in COPD rats based on TGF-β1/Smads pathway
Objective To explore the intervention effect and mechanism of Guilu Bushen Wan on airway remodeling in rats with chronic obstructive pulmonary disease(COPD).Methods Sixty SPF SD rats were randomly divided into normal control group,model group,and low,medium,and high doses of Guilu Bushen Wan groups(1.8 g/kg,3.6 g/kg,7.2 g/kg),and aminophylline group(30.0 mg/kg).The COPD model was replicated by intratracheal infusion of lipopolysaccharide combined with smoking.Starting from the 29th day,the normal control group and model group were given physiological saline by gavage,while the other treatment groups were given corresponding drugs for 28 consecutive days.On the 57th day of the experiment,lung function and pathological changes in lung tissue were detected.ELISA was used to detect the levels of MMP-9,MMP-12,and TIMP-1 in bronchoalveolar lavage fluid(BALF).IHC method was used to detect the expression of TGF-β1,COL-I and α-SMA in lung tissue.Western blot was used to detect the expression of TGF-β1,p-Samd2 and p-Samd3 in lung tissue.Results Compared with the control group,PEF,PIF,MV and EF50 of lung function were decreased in the model group.The infiltration of inflammatory cells in lung tissue was obvious,MAN value was decreased,and MLI value and the deposition of airway collagen fiber were significantly increased.The contents of MMP-12 and TIMP-1 in BALF were increased,and the expressions of TGF-β1,p-Samd2,p-Samd3,COL-I and α-SMA in lung tissue were upregulated.After treatment with Guilu Bushen Wan,PIF,PEF,MV and EF50 were increased.The infiltration of inflammatory cells in lung tissue was reduced,MAN value was increased,and MLI value and the deposition of airway collagen fiber were lowered.The contents of MMP-12 and TIMP-1 in BALF and the expression of TGF-β1,p-Samd2,p-Samd3,COL-I and α-SMA in lung tissue were decreased.Conclusion Guilu Bushen Wan can improve lung function and reduce airway remodeling in COPD rats,which may be related to regulating TGF-β1/Smads signaling pathway.
万方数据
维普
