摘要
目的 建立麦门冬汤基准样品的指纹图谱并结合化学模式识别法进行评价,同时建立多指标成分含量测定方法.方法 采用超高效液相色谱-蒸发光散射检测器(UPLC-ELSD)和UPLC-二极管阵列检测器(PDA)建立麦门冬汤的指纹图谱,明确共有峰归属并进行相似度分析、聚类分析、主成分分析、正交偏最小二乘判别分析等化学模式识别研究.测定样品中10种指标性成分的质量分数.结果 建立麦门冬汤基准样品2套指纹图谱方法,相似度均大于0.90,标定了24个共有峰,分别来自方中麦冬、甘草、人参3个药味;化学模式识别法将20批样品分为5类,筛选出影响不同批次基准样品质量差异的5种关键成分;甲基麦冬二氢高异黄酮A和甲基麦冬二氢高异黄酮B、麦冬皂苷D和麦冬皂苷C、麦冬龙脑苷、人参皂苷Rg1和人参皂苷Re、人参皂苷Rb1、甘草苷、甘草酸质量分数均值的±30%范围分别为0.001%～0.003%、0.002%～0.004%、0.003%～0.005%、0.04%～0.08%、0.02%～0.03%、0.08%～0.16%、0.06%～0.11%.结论 建立的麦门冬汤基准样品质量分析方法科学、准确且稳定,可为经典名方麦门冬汤相关制剂的开发研究及其质量控制提供参考依据.
Abstract
Objective To establish the fingerprint of the benchmark sample of Maimendong decoction by chemical pattern recognition and establish a multi-index component content determination method.Methods Ultra-performance liquid chromatography-evaporative light scattering detector(UPLC-ELSD)and UPLC-diode array detector(PDA)were used to establish the fingerprint of Maimendong decoction,the common peak assignment was clarified,and chemical pattern recognition was carried out by similarity analysis,cluster analysis,principal component analysis,orthogonal partial least squares discriminant analysis,etc.The content of 10 index components in the samples was determined.Results Two sets of fingerprint methods were established for the reference samples of Maimendong decoction,with similarity greater than 0.90.24 common peaks were calibrated,which were from Maidong,Licorice,and Ginseng in the formula.The chemical pattern recognition method divided 20 batches of samples into 5 categories and selected 5 key components that affected the quality differences of benchmark samples from different batches.The±30%ranges of the average content of methylophiopogonanone A and methylophiopogonanone B,ophiopogonin D and ophiopojaponin C;borneol 7-O-[β-D-apiofuranosyl-(1→6)]-β-D-glucopyranoside,ginsenoside Rg1 and ginsenoside Re,ginsenoside Rb1,liquiritin,glycyrrhizicacidis were 0.001%-0.003%,0.002%-0.004%,0.003%-0.005%,0.04%-0.08%,0.02%-0.03%,0.08%-0.16%,and 0.06%-0.11%,respectively.Conclusion The establishment of a scientific,accurate and stable method for the quality analysis of benchmark samples of Maimendong decoction can provide a basis for the development,research and quality control of related preparations of the classic famous Maimendong decoction.
基金项目
澳门特别行政区科学技术发展基金(0001120201AKP)
佛山市核心攻关项目(1920001000378)
佛山市南海区重点领域科技攻关专项(2022)(南科[2023]20号)
维普
万方数据