Rapid analysis components of Tuihuang Mixture based on UHPLC-Q-Exactive Orbitrap MS
Objective An effective and rapid analysis method by UHPLC-Q-Exactive Orbitrap MS was established to identify the components of Tuihuang Mixture.Methods The analysis was performed on a Waters ACQUITY UPLC BEH C18(100 mm×2.1 mm,1.7 µm)HPLC column.The mobile phase consisted of 0.1%formic acid(A)and acetonitrile(B)was used as gradient elute.The flow rate was 0.3 mL·min-1,column temperature was 40℃,and injection volume was 2 μL.The ESI was operated in negative mode with the scanning range of m/z 100～1500.Results A total of 77 chemical components were identified by standards,database and references,including 30 flavonoid compounds,18 polyphenol compounds,10 organic acid compounds,9 terpenoids compounds,9 anthraquinone compounds,1 other constituent.Conclusion The established UHPLC-Q-Exactive Orbitrap MS technology can rapidly analyze and identify the components of Tuihuang Mixture.
万方数据
维普
