Effects of salt stress and drought stress on Andrographis paniculata seed germination characteristics
Objective To study the effects of salt stress and drought stress on the germination characteristics of Andrographis paniculata seeds.Methods Eight A.paniculata(Burm.f.)Nees seeds from different sources were treated with 0%,0.05%,0.10%,0.20%,0.40%NaCl solution,and the germination potential,germination rate,germination index,radicle and germ length and vitality index were measured.The salt resistance of different A.paniculata(Burm.f.)Nees germplasm was evaluated by membership function analysis.The A.paniculata(Burm.f.)Nees seeds collected from Yulin base,Guangxi were treated with 0,5%,10%,15%,20%and 25%PEG-600,and the germination potential,germination rate,germination index,radicle and germ length,activity index,SOD,POD,CAT enzyme activity,and contents of soluble protein and malondialdehyde were determined.Results With the increase of salt concentration,the germination potential,germination rate,germination inde,radicle germ length and vitality index of A.paniculata seeds of 8 germplasm decreased with the increase of salinity,and the environment with salinity above 0.20%was not conducive to the germination of A.paniculata(Burm.f.)Nees seeds.Membership function analysis showed that the salt tolerance of 8 germplasm from different sources was as follows:Yingde City,Guangdong Province>Zhanjiang City,Guangdong Province(conventional),Zhanjiang City,Guangdong Province(large leaf)>Zhangzhou City,Fujian Province>Nanning City,Guangxi Province>Guigang City,Guangxi Province>Yulin City,Guangxi Province>Chengdu City,Sichuan Province.With the increase of drought degree,the germination potential,germination rate,germination index,radicle germ length and activity index of A.paniculata(Burm.f.)Nees seeds decreased and the content of malondialdehyde increased,but under 5%PEG-6000,the activity of SOD,POD and CAT enzymes and the content of soluble protein increased.Conclusion Salt and drought stress has great influence on the germination characteristics of A.paniculata(Burm.f.)Nees.A.paniculata(Burm.f.)Nees seeds have certain salt tolerance and the salt tolerance is different in different places,A.paniculata(Burm.f.)Nees from Guangdong and Fujian have good salt resistance.When the water potential is lower than-0.026MPa(5%PEG-6000),water should be added in time during the cultivation of A.paniculata(Burm.f.)Nees seedlings.
万方数据
维普
