Proximity labeling(PL)technology refers to the in-situ labeling of protein-interacting proteins of interest by fusing specific catalytic enzymes,followed by mass spectrometry or sequencing analysis to identify the tagged proteins.This technique can accurately identify protein-protein interactions under transient dynamic condition,which is an important supplement to traditional analytical methods,and has a broad application prospect in the study of protein-protein interactions.In this paper,the working principle,classification,advantages and disadvantages of various PL enzymes were introduced,then the application of this technology in the study of protein-protein interactions was discussed,and finally the advantages,existing problems and development trends of adjacent labeling technology were discussed,in order to provide reference for the application of this technology.
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