Study on the spectrum effect relationship between high performance liquid chromatography fingerprint and antioxidant activity of Polygonum bistorta L.
Objective The high performance liquid chromatography(HPLC)fingerprints were correlated with the antioxidant activity to establish a spectrum-effect relationship to screen the active ingredients with the antioxidant activities of Polygonum bistorta L.Methods The fingerprint of P.bistorta L.was established by HPLC and the similarity of the fingerprint was evaluated.Combined with HPLC-Q-Orbitrap HRMS/MS qualitative analysis,29 of the major characteristic peaks were designated.The antioxidant activity of different batches was evaluated by the free radical scavenging rate of 2,2′-diazo-bis(3-ethylbenzothiazolin-6-sulfonic acid)diamine salt(ABTS),2,2-diphenyl-1-trinitrophenylhydrazine(DPPH),and total antioxidant capacity(FRAP).The antioxidant activity comprehensive index(APC)was used for a comprehensive evaluation.Grey relational analysis and partial least square regression analysis were used for a comprehensive evaluation.Results The HPLC fingerprints of 11 samples were established,with the similarity greater than or equal to 0.970,demarcating 29 main characteristic peaks,and identifying 26 components.The results of grey relational analysis and partial least square regression analysis showed that peaks 2+3(6-O-Galloylglucose),8(Digallic glucose),11(Chlorogenic acid),14(Catechin),15(Digallic glucose),18(Digallic glucose),and 23(Epicatechin)were the important characteristic peaks reflecting the antioxidant efficacy of P.bistorta L.Conclusion The antioxidant activity of P.bistorta L.is the result of the combined effect of multi-components such as phenolic acids and flavonoids,which provides a more comprehensive reference for the quality control of medicinal materials.
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