Study on the quality standard of Hypericum japonicum Thunb formula granules based on standard decoction
Objective The quality standard of the formula granules was established based on the standard decoction of Hypericum japonicum Thunb to provide reference for the quality evaluation of the formula granules.Methods The contents of quercetin and isoquercetin in decoction pieces,standard decoction and formula granules were determined by high performance liquid chromatography(HPLC),and the transfer rates of the two components were calculated.The analysis method of standard decoction and formula granules was established by HPLC.Results The contents of quercetin and isoquercetin in 15 batches of standard decoction were 13.66-32.11 mg/g,4.00-16.81 mg/g,the transfer rates were 45.7%-63.5%,37.8%-56.9%.The contents of quercetin in 3 batches of formula granules were 16.16,17.02 and 16.52 mg/g,the transfer rate was 55.2%,58.2%,55.3%.The isoquercitrin content was 7.61,7.93,7.56 mg/g,and the transfer rate was 45.0%,48.8%and 44.7%.The similarity between the characteristic maps of each sample and the control maps was greater than 0.90.The established characteristic maps identified a total of 7 peaks,of which 4 were identified.Peak 3 was isoquercetin,peak 5 was quercetin,peak 6 was leucoside B and peak 7 was quercetin.It was divided into two categories,the cumulative variance contribution of the two principal components was 84.85%.Conclusion The established HPLC method of content determination and characteristic map is specific,reproducible,reasonable and accurate,and the obtained standard decoction and formula granules are in good consistency,which can provide a reference for the quality control and overall evaluation of the formula granules.
万方数据
维普
