Simultaneous determination of four components in Sujie Oral Liquid by UHPLC-MS/MS
Objective To establish a method for the simultaneous and rapid determination of four components,i.e.liquiritin,rosmarinic acid,platycodon D and glycyrrhizic acid in Sujie Oral Liquid by ultra high performance liquid chromatography-tandem quadrupole mass spectrometry(UHPLC-MS/MS),so as to control the quality of Sujie Oral Liquid.Methods The samples were separated on an Agilent Extend C18(2.1 mm×100 mm,1.7 μm)column with a mobile phase containing 0.1%formic acid(A)and acetonitrile(B)by gradient elution.The column temperature was set at 35℃,and the flow rate was 0.3 mL/min.ESI ion source was used.In multiple reaction monitoring mode,they were analyzed in negative ion mode.The ion pairs were as follows:liquiritin 417.1/255.1(135),rosmarinic acid 359/197.1(160.9),platycodon D 1 223.6/681.0,glycyrrhizic acid 821.4/350.9.Results The four components showed good linear relationships within their mass concentration ranges(r>0.999),whose average recoveries were 98.80%-100.84%with the RSDs of 1.82%-2.53%.In 10 batches of Sujie Oral Liquids,the mass concentrations of liquiritin,rosmarinic acid,platycodon D,and glycyrrhizic acid were 0.140 8-0.159 2,0.082 4-0.142 1,0.035 9-0.069 8,and 0.665 9-0.723 8 mg/mL,respectively.Conclusion The method of simultaneous determination for four components in Sujie Oral Liquid is fast,accurate and highly reproducible,which provides experimental basis for the quality evaluation of Sujie Oral Liquid.
万方数据
维普
