Xuantong prescription improves post-stroke depression via regulating monoamine neurotransmitter and oxidative stress
Objective To explore the active components of Xuantong prescription and its mechanism in the treatment of post-stroke depression(PSD)rats.Methods UPLC-Q-TOF-MS technology was used to identify the active components of Xuantong prescription.Based on the identified components,the potential mechanism of Xuantong prescription in the treatment of PSD was predicted by network pharmacological analysis.The PSD rat model was constructed,and the pharmacodynamic effect of Xuantong prescription on PSD rats was explored by behavioral experiments and pathological tissue staining,and the effects of Xuantong prescription on monoamine neurotransmitters and oxidative stress levels in PSD rats were detected by ELISA.Results Totally 57 active components of Xuantong prescription,734 potential targets,and 562 intersected with PSD-related targets were obtained.Common targets were mainly enriched in monoamine neurotransmitter pathways,oxidative stress responses and other processes.The in vivo experimental results indicated that compared with the model group,the sucrose preference rate of rats in the escitalopram group and high-dose Xuantong prescription group significantly increased(P<0.01,P<0.05),the number of open field test activities significantly increased(P<0.01,P<0.05),the tail suspension time significantly decreased(P<0.05,P<0.01),and the forced swimming immobility time significantly decreased(P<0.05).In each administration group,the arrangement of hippocampal tissue was relatively orderly,pathological changes were obviously mitigated,and the number of neurons and Nissl-positive cells were higher when compared to the model group.In the group of rats treated with high-dose Xuantong prescription,there was a significant increase in serum levels of 5-HT(P<0.05),DA(P<0.01,P<0.05),antioxidant enzyme SOD(P<0.01,P<0.05),and CAT(P<0.01),along with a significant decrease in lipid peroxide MDA levels(P<0.01).Conclusion Xuantong prescription can play a role in the treatment of PSD by regulating monoamine neurotransmitters and oxidative stress.
万方数据
维普
