Study on quality standard of Pueraria(Kudzu)formula granules based on standard decoction
Objective To establish the quality standard of Pueraria(Kudzu)formula granules based on the study of standard decoction.Methods HPLC was used to determine the content of radioglycoside in decoction pieces,standard decoction and formula granules,and the transfer rate was calculated.HPLC chromatographic analysis and TLC identification of standard decoction and formula granules were established.Results The mass fraction of radioglycoside in 19 batches of Kudzu decoction pieces was 0.46%-2.19%.The mass fraction of radioglycoside in standard decoction was 7.4-26.3 mg/g,and the transfer rate was 31.2%-55.7%.The mass fraction of 3 batches of formula granules was 14.3,12.6,10.5 mg/g,respectively,and the transfer rates were 36.3%,37.6%and 32.2%,respectively.There were 5 common peaks in the HPLC characteristic maps of 19 batches of standard decoction,and 5 components of 6″-O-xylososide,dzein,iristin-7-O-xyloglucoside,genistein and radioglycoside were identified.The similarity of the characteristic maps was greater than 0.90,and the clustering was 4 classes.The cumulative variance contribution rate of the two principal components was 81.963%.There were also 5 common characteristic peaks in the HPLC chromatograms of the three batches of formula granules,and the similarity was 0.979,0.981,0.985,respectively.It could provide rich information,clear spots and good separation with TLC.Conclusion The established method is stable and reasonable,which can be used to evaluate the quality of the formula granules,and provide a basis for its quality control.
万方数据
维普
