Combining network pharmacology and molecular docking to investigate the effect mechanism of Chinese medicine sachet on a chronic restraint stress-induced anxiety model in mice
Objective To explore the intervention effect and mechanism of a Chinese medicine sachet on anxiety-like behavior in anxiety model mice.Methods The chronic restraint stress(CRS)method was used to establish an anxiety mouse model.Open field test and elevated plus maze investigated the effect of the Chinese medicine sachet on anxiety-like behavior in mice.The levels of 5-hydroxytryptamine(5-HT)and corticosterone(CORT)in the hippocampus and serum of mice were detected by ELISA.The targets of Chinese medicine sachet compounds were mined from the TCMSP platform,and the targets of anxiety diseases were obtained from OMIM,GeneCards,and DisGeNET databases.The Venny platform was used to obtain the component-disease intersection targets for GO and KEGG enrichment.The active ingredient-target-pathway network was constructed by Cytoscape.Active ingredients and key targets with larger Degree values were selected for molecular docking validation.Results Chinese medicine sachet significantly improved the anxiety-like behavior of CRS mice,increased the level of 5-HT in the hippocampus and serum,and reduced the level of CORT in serum.Network pharmacology showed that multiple active ingredients(acacetin,naringenin,wogonin,etc.)in the Chinese medicine sachet act on various key targets(AKT1,SRC,ESR1,CASP3,etc.)and regulate multiple signaling pathways(cancer pathway,PI3K-AKT signaling pathway,etc.).The molecular docking results showed that the active ingredients(senkyunone,acacetin,daucosterol,genkwanin,naringenin,and wogonin)have high binding activities to the key targets(SRC,ESR1,CASP3,and EGFR).Conclusion The Chinese medicine sachet can improve anxiety-like behavior and levels of 5-HT and CORT in CRS mice.It can intervene in anxiety through multiple components,targets,and pathways,providing direction for future mechanism research.
Chinese medicine sachetanxietychronic restraint stress modelnetwork pharmacologymolecular docking
万方数据
维普
