Correlation analysis of vitamin D receptor single nucleotide polymorphisms and susceptibility to hypertensive disorders of pregnancy
Objective To explore the correlation between vitmin D receptor(VDR)single nucleotide polymorphism(SNP)and susceptibility to hypertensive disorders of pregnancy(HDP).Methods A total of 122 pregnant women with HDP attending Yan'an Hospital of Kunming City from September 2019 to October 2022 were selected as the HDP group,and another 122 pregnant women with normal pregnancies during the same period were selected as the control group.The SNPs of rs11568820,rs2228570 and rs1544410 were determined by TaqMan probe and PCR instrument.The chain disequilibrium(LD)and Hardy-Weinberg equilibrium(HWE)of SNPs were analyzed.Clinical data,genotypes and allele distribution of pregnant women in the two groups were compared and analyzed.Results The results of VDR genotyping were consistent with the sequencing results.There was no strong chaining phenomenon among the three loci and followed HWE(P＞0.05).Compared with the control group,the rs2228570 gene had a higher proportion of both GG type and G allele in the HDP group,and the difference was statistically significant(x2=6.799 and 4.066,respectively,P＜0.05).Both rs11568820 and rs1544410 genes were not associated with the risk of HDP(P＞0.05).Further stratified analysis of the association between the rs2228570 locus and the development of HDP showed that a higher proportion of pregnant women with triglyceride(TG)≥3.82 mmol/L,1,25-(OH)2D3＜20.18 nmol/L,and creatinine(Cr)≥68.78μmol/L were found in the GG genotype group compared to AG/AA genotypes,with OR values and 95％CI were 1.555(1.320-1.927),2.402(1.215-2.734),and 1.902(1.267-2.303),respectively.Conclusion Carrying the G allele at the rs2228570 locus of the VDR gene increases the risk of HDP and is closely associated with high TG,Cr levels,and low 1,25-(OH)2D3 levels.No association is found between rs11568820,rs1544410 and HDP susceptibility.
vitamin D receptorgene polymorphismhypertensive disorders of pregnancysusceptibilitycorrelation
万方数据
维普
