Relationship between vaginal flora dysbiosis and the risk of early pregnancy abortion
Objective To explore the relationship between vaginal flora dysbiosis and the risk of early pregnancy abortion.Methods From May,2021 to March,2022,a total of 147 pregnant women in Department of Gynecology,The Second Affiliated Hospital of Shaanxi University of Chinese Medicine participated in this study.Among them,the case group consisted of patients with early pregnancy obortion(diagnosed within 12 gestation weeks)(n=64),and the control group consisted of healthy full-term delivery women(n=83).The composition of vaginal microbiota in early pregnancy was compared and analyzed between the case group and the control group using 16S rRNA gene sequencing.Results There were no statistically significant differences in age,body mass index,number of smokers,pregnancy status,previous abortions,gestational age,and vaginal pH between the case group and the control group(P＞0.05).A total of 244 bacterial species were identified in the sample cohort.The samples were divided into 5 major types,among which the characteristic of community state type(CST)Ⅰ was dominance of Lactobacillus crispatus,CST Ⅱ was dominance of Lactobacillus gasseri,CST Ⅲ was dominance of Lactobacillus iners,CST Ⅴ was dominance of Lactobacillus jensenii,and CST Ⅳ had a lower abundance of Lactobacillus species.The highest α-diversity and abundance were observed in CST Ⅳ communities.Compared to the control group,the proportion of Lactobacillus consumption in the vaginal microbiota composition was significantly increased in the case group[25.0％(16/64)vs.6.0％(5/83),x2=10.626,P＜0.01].The proportion of CST Ⅳtype as the dominant vaginal microbiota was significantly increased in the case group[29.7％(19/64)vs.12.0％(10/83),x2=7.100,P＜0.01].The Simpson's diversity index(2.81±1.20 vs.1.41±0.48)and abundance(28.06±15.08 vs.17.13±6.83)were significantly higher in the case group compared to the control group(t=4.641 and 3.523,respectively,P＜0.05).This correlation persisted when pregnant women with vaginal bleeding scores＞1 were excluded from the case group.Compared to missed abortion,the proportion of Lactobacillus consumption in the vaginal microbiota composition of incomplete/complete abortion was significantly increased[19.2％(10/52)vs.75.0％(9/12),x2=14.527,P＜0.01].The proportion of CST Ⅳ type as the dominant vaginal microbiota was significantly increased in incomplete/complete abortion[23.1％(12/52)vs.75.0％(9/12),x2=11.923,P＜0.01].Conclusions The high diversity of vaginal bacterial community and the decrease of Lactobacillus are the risk factors for early pregnancy abortion.
万方数据
维普
