Objective To analyze the expression and significance of human fibrinogen-like protein(Hfgl2)in cervical cancer tissues and explore its impact on the malignant biological behavior of cervical cancer.Methods We selected tumor specimens from 50 cervical cancer patients diagnosed pathologically at the First Hospital of Yulin from January 2016 to January 2018 as the experimental group,and collected 50 normal cervical tissue specimens from the same period as the control group.Immunohistochemistry and reverse transcription-polymerase chain reaction(RT-PCR)were used to detect tissue samples.Western blot(WB)was employed to assess Hfgl2 expression in cells,and the relationship with clinical pathological characteristics of cervical cancer was analyzed.Gene knockdown was utilized to downregulate Hfgl2 expression,and the methylthiazolyldiphenyl-tetrazolium bromide(MTT)assay was employed to measure cell proliferation ability,with WB used to evaluate the expression of proteins related to angiogenesis,cell proliferation,and cell migration.Results The positive expression rate of Hfgl2 in cervical cancer tissues was higher than that in normal cervical tissues,with statistically significant differences(x2=73.990,P＜0.05);The immunohistochemical score of Hfgl2 in cervical cancer tissues was higher than those in normal cervical tissues,with statistically significant differences(t=8.022,P＜0.05);RT-PCR analysis revealed a higher positive rate of Hfgl2 mRNA expression in the experimental group compared to the control group,with statistically significant differences(x2=61.455,P＜0.05);The positive expression rate of Hfgl2 in Federation International of Gynecology and Obstetrics(FIGO)stage Ⅲ-Ⅳ cervical cancer was higher than in stage Ⅰ andⅡ groups,higher in pathological grade G2-G3 cervical cancer than in G1,and higher in cervical cancer tissues with lymph node metastasis than in those without,all with statistically significant differences(x2=6.737,7.147,and 12.037 respectively,P＜0.05);Hfgl2 was highly expressed in cervical cancer cell lines,and knockdown of Hfgl2 resulted in a significant decrease in cell vitro proliferation ability,and the expression of angiogenesis and proliferation-related proteins vascular endothelial growth factor receptor 1(VEGFR1),protein kinase B(PKB/Akt),phospholipase C(PLC)-γ,matrix metalloproteinase(MMP)2,and MMP9 in human cervical cancer cells HeLa were significantly downregulated,with statistically significant differences(t=4.904-8.304,P＜0.05).Conclusion Hfgl2 is highly expressed in cervical cancer tissue and may promote tumor angiogenesis and invasive growth,which is expected to become a new direction for targeted treatment in cervical cancer.
关键词
人纤维介素/宫颈癌/临床病理特征/血管生成/增殖迁移
Key words
human fibrinogen-like protein 2/cervical cancer/clinical pathological characteristics/angiogenesis/proliferative and migration
维普
万方数据