Application value of serum miR-182-5p,CFB and ADAM12 detection in prenatal screening for Down's syndrome in early pregnancy
Objective To investigate application value of detection for serum levels of micro ribonucleic acid(miR)-182-5p,complement factor B(CFB)and a disintegrin and metalloprotease 12(ADAM12)in early pregnancy in prenatal Down's syndrome(DS)screening.Methods 150 pregnant women with DS fetuses who admitted to Ya'an Municipal People's Hospital from January 2015 to January 2022 were selected as the DS group,and another 87 pregnant women with healthy fetuses in the same period were selected as the control group.Real-time fluorescence quantitative polymerase chain reaction(qPCR)was used to detect serum miR-182-5p level,and enzyme-linked immunosorbent assay(ELISA)was used to detect serum CFB and ADAM12 levels.Multifactorial Logistic regression was used to analyze influencing factors of DS and receiver operating characteristic(ROC)curves was used to analyze predictive values of serum miR-182-5p,CFB and ADAM12 levels in early pregnancy for DS.Results The pregnant women in the DS group were older than those pregnant women in the control group,their proportion of smoking and serum CFB level were higher than those in the control group,while the serum levels of miR-182-5p and ADAM12 were lower than those in the control group,and the differences were statistically significant(x2/t/Z values ranged from 3.141 to 8.793,all P＜0.05).Multifactorial Logistic regression analysis showed that increase in age,smoking and elevated serum CFB level were independent risk factors for DS,while elevated serum miR-182-5p and ADAM12 levels were independent protective factors for DS,and their ORs(95％CIs)were 1.122(1.012-1.243),4.290(1.216-15.131),1.460(1.268-1.681),0.656(0.563-0.765)and 0.996(0.994-0.998)respectively,all P＜0.05.Receiver operating characteristic(ROC)curve analysis showed that the AUC of the combined prediction of serum miR-182-5p,CFB and ADAM12 levels in early pregnancy for DS was 0.913,which was greater than those predicted by each index alone(Z=5.233,4.414 and 4.809 respectively,all P＜0.05).Conclusion Detection of maternal serum miR-182-5p,CFB and ADAM12 levels has potential application values in prenatal screening for DS in early pregnancy,and the combination of all three is beneficial in screening for DS in early pregnancy.
early pregnancyDown's syndromemicro ribonucleic acid-182-5pcomplement factor Ba disintegrin and metalloprotease 12prenatal screening
万方数据
维普
