The correlation between the expression levels of serum TIM-4 and HBD2 and the condition of children with Mycoplasma pneumoniae pneumonia
Objective To explore the correlation between the expression levels of serum T cell immunoglobulin and mucin domain molecule-4(TIM-4)and human β-defensin 2(HBD2)and the disease of children with Mycoplasma pneumoniae pneumonia(MPP).Methods A total of 142 children with MPP admitted to our hospital from July 2020 to January 2023 were collected as the study subjects,and according to the severity of their diseases,they were divided into the ordinary MPP group(n=99)and the refractory MPP group(n=43),and 140 children with healthy physical examination during the same period were selected as the control group.Enzyme linked immunosorbent assay(ELISA)was applied to detect serum TIM-4 and HBD2 levels;the clinical data of pediatric patients were collected,and the influencing factors of refractory MPP were analyzed by multivariate logistic regression analysis;receiver operating characteristic(ROC)curves were applied to analyze the diagnostic value of serum TIM-4 and HBD2 levels in refractory MPP.Results There were statistically significant differences between the ordinary MPP group and the refractory MPP group in terms of fever duration,hospital stay,azithromycin treatment time,neutrophil percentage,white blood cell count,recurrent respiratory tract infections,and extrapulmonary complications(t/x2 value ranged from 2.195-21.265,P＜0.05);compared with the control group,the expression levels of serum TIM-4 and HBD2 in the study group were significantly higher(t=34.527 and 40.646,respectively,P＜0.05);compared with the ordinary MPP group,the expression levels of serum TIM-4 and HBD2 in the refractory MPP group were significantly higher(t=12.410 and 15.316,respectively,P＜0.001).The results of multivariate logistic regression analysis showed that serum TIM-4 level(OR=1.423,95％CI:1.140-1.776),HBD2 level(OR=1.436,95％CI:1.162-1.775),fever duration(OR=1.349,95％CI:1.056-1.724),neutrophil percentage(OR=1.334,95％CI:1.032-1.725),recurrent respiratory infections(OR=1.521,95％CI:1.245-1.858),and extrapulmonary complications(OR=1.432,95％CI:1.152-1.780)were all independent risk factors for the development of refractory MPP(P＜0.05).The ROC results showed that the area under the curve(AUC)of serum TIM-4 level in the diagnosis of refractory MPP alone was 0.845(95％CI:0.774-0.900),and the sensitivity and specificity were 69.77％and 85.86％,respectively;the AUC of serum HBD2 level alone in the diagnosis of refractory MPP was 0.815(95％CI:0.742-0.875),and the sensitivity and specificity were 74.42％and 82.83％,respectively;the AUC of refractory MPP diagnosed by the combination of the two was 0.908(95％CI:0.848-0.950),and the sensitivity and specificity were 90.70％and 81.82％,respectively,which was significantly higher than those of serum TIM-4 level alone(Z=2.047,P=0.040)and serum HBD2 level alone(Z=2.443,P=0.015).Conclusion The expression of serum TIM-4 and HBD2 is increased in children with Mycoplasma pneumoniae pneumonia,and their levels are elevated in the diagnosis of refractory Mycoplasma pneumoniae pneumonia.
万方数据
维普
