Serum levels of miR-20b and miR-4443 of patients with severe preeclampsia and their relationships with adverse pregnancy outcomes
Objective To detect serum levels of microRNA-20b(miR-20b)and microRNA-4443(miR-4443)of patients with severe preeclampsia,and to explore their predictive values for pregnancy outcomes of the patients.Methods A total of 106 patients with severe preeclampsia who were treated in our hospital from February 2021 to January 2023 were selected as severe preeclampsia group,and 106 patients with mild preeclampsia were selected as mild preeclampsia group,other 106 healthy pregnant women who received regular prenatal examination in the same period were as the control group.Quantitative real-time PCR(qRT-PCR)method was applied to detect expression levels of miR-20b and miR-4443 in serum.According to pregnancy outcome,the patients with preeclampsia were divided into good pregnancy outcome group(n=55)and poor pregnancy outcome group(n=51).Multivariate Logistic regression was applied to analyze influencing factors of pregnancy outcomes of the patients with severe preeclampsia.Receiver operating characteristic(ROC)curve was applied to evaluate values of miR-20b and miR-4443 in predicting adverse pregnancy outcomes of the patients with severe preeclampsia.Results The serum levels of miR-20b and miR-4443 of the pregnant women in the control group,mild preeclampsia group and severe preeclampsia group gradually increased(F=486.199 and 354.434 respectively,both P＜0.001).There were significant differences in incidences of fetal intrauterine distress and preterm delivery(x2=10.498 and 15.163 respectively,both P＜0.001).The total incidence of adverse pregnancy outcomes in the severe preeclampsia group was 48.11％,which was significantly higher than those in the mild preeclampsia group and the control group(x2=50.956,P＜0.001).The serum levels of miR-20b and miR-4443 of the pregnant women in the poor pregnancy outcome group were significantly higher than those in the good pregnancy outcome group(t=5.750 and 5.853 respectively,both P＜0.001).Multivariate Logistic regression analysis showed that miR-20b(OR=3.564,95％CI:1.546-8.214),miR-4443(OR=2.365,95％CI:1.472-3.800),systolic blood pressure(OR=2.869,95％CI:1.373-5.995),urinary protein(OR=3.837,95％CI:1.910-7.709)and triglycerides(TG)(OR=4.236,95％CI:2.027-8.851)were all risk factors for adverse pregnancy outcomes of the patients with severe preeclampsia(all P＜0.05).ROC curve analysis showed that area under the curve(AUC)of combination detection of serum miR-20b and miR-4443 levels for predicting pregnancy outcome of the patients with severe preeclampsia was 0.945(95％CI:0.901-0.989),which was significantly higher than those predicted by single detection of miR-20b(Z=2.412,P＜0.05)and miR-4443 alone(Z=2.055,P＜0.05).Conclusion The miR-20b and miR-4443 of the patients with severe preeclampsia are highly expressed in serum,which is related to risk of adverse pregnancy outcomes.The combination detection of serum miR-20b and miR-4443 levels can be considered as a predictive index for adverse pregnancy outcomes of the patients with severe preeclampsia.
severe preeclampsiamicro RNA-20bmicro RNA-4443pregnancy outcomeprediction
万方数据
维普
