The relationship between MYLIP and miR-542-3p with early diagnosis and clinical prognosis in patients with cervical cancer
Objective To investigate the value of serum myosin regulatory light chain interacting protein(MYLIP)and microRNA-542-3p(miR-542-3p)expression levels in the early diagnosis of cervical cancer(CC)and their relationship with clinical prognosis.Methods A total of 128 patients with CC admitted to our hospital from May 2018 to May 2020 were selected as the CC group,86 patients with cervical intraepithelial neoplasia(CIN)who were treated in our hospital during the same period were selected as the CIN group,and 74 healthy patients who underwent physical examination were selected as the control group.The expression levels of serum MYLIP mRNA and miR-542-3p in each group were detected by real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR),and the clinicopathological characteristics of CC patients were collected,and the relationship between the expression levels and the clinicopathological characteristics of CC patients was analyzed.The ENCORI website was used to predict whether there was a targeting relationship between MYLIP and miR-542-3p;Pearson method was applied to analyze the correlation between MYLIP mRNA and miR-542-3p expression;the receiver operating characteristic(ROC)curve was plotted to evaluate the value of MYLIP mRNA and miR-542-3p expression levels in the early diagnosis of CC;the Kaplan-Meier method was applied to analyze the relationship between serum MYLIP mRNA and miR-542-3p expression levels and the prognosis of CC patients;Cox regression was used to analyze the risk factors affecting the prognosis and mortality in patients with CC.Results The expression level of serum MYLIP mRNA in CC group was significantly higher than that in CIN group and the control group(F=118.850,P＜0.001),and the expression level of miR-542-3p was significantly lower than that in CIN group and the control group(F=85.413,P＜0.001).the expression levels of serum MYLIP mRNA and miR-542-3p in CC patients were correlated with menopause(x2=5.02 6,6.13 3),depth of cervical infiltration(x2=6.767,7.957),FIGO stage(x2=7.788,11.480),TNM stage(x2=12.426,20.150),and lymph node metastasis(x2=8.821,12.687)(P＜0.05);there were targeted binding sites between MYLIP and miR-542-3p,and serum MYLIP mRNA was negatively correlated with miR-542-3p expression in CC patients(r=-0.415,P＜0.001).The AUC of serum MYLIP mRNA and miR-542-3p expression levels individually and in combination in the diagnosis of CC were 0.807,0.815,and 0.894,the combined diagnosis effect was better than that of individual diagnosis(Z the two are combined-MYLIP=2.165,P=0.030;Zthe two are combined-miR-542-3P=2.360,P=0.018).The 3-year survival rate of patients with high expression of serum MYLIP mRNA was significantly lower than that of patients with low expression(52.24％vs.78.69％,x2=10.869,P=0.001),and the 3-year survival rate of patients with high expression of serum miR-542-3p was significantly higher than that of patients with low expression(79.03％vs.51.52％,x2=11.771,P=0.001).High expression of MYLIP mRNA is a risk factor for death in CC patients(HR=1.972,95％CI:1.565-2.485),and high expression of miR-542-3p is a protective factor for death in CC patients(HR=0.517,95％CI:0.399-0.670)(P＜0.05).Conclusion The expression levels of serum MYLIP mRNA and miR-542-3p have certain value in the early diagnosis of cervical cancer,and their expression levels are closely related to the clinical prognosis of cervical cancer patients.
万方数据
维普
