The relationship between serum IGF2BP2 expression and insulin resistance in patients with polycystic ovary syndrome
Objective To analyze the relationship between serum insulin-like growth factor 2 mRNA binding protein 2(IGF2BP2)and fibroblast growth factor 21(FGF-21)expression and insulin resistance(IR)in patients with polycystic ovary syndrome(PCOS).Methods A total of 136 patients with PCOS admitted to Jianyang Chinese Medicine Hospital from December 2021 to May 2023 were selected as the experimental group,and 70 healthy individuals undergoing physical examinations during the same period as the control group.Based on the insulin resistance index(HOMA-IR),the PCOS patients were divided into an IR group(91 cases)and a non-IR group(45 cases).Serum IGF2BP2 levels were measured by quantitative real-time polymerase chain reaction(qRT-PCR),and serum FGF-21 levels were detected by enzyme-linked immunosorbent assay(ELISA).Spearman correlation analysis was conducted,logistic regression analysis was used to identify factors influencing IR,and receiver operating characteristic(ROC)curve analysis assessed the value of serum IGF2BP2 and FGF-21 levels in predicting IR in PCOS patients.Results The serum levels of IGF2BP2 and FGF-21 in PCOS patients were higher than those in healthy individuals,and the difference was statistically significant(t=8.271 and 9.438,respectively,P＜0.05).The serum levels of IGF2BP2 and FGF-21 of PCOS patients in the IR group were also higher than those in the non-IR group,and the difference was statistically significant(t=5.432 and 7.323,respectively,P＜0.05).A positive correlation was observed between serum IGF2BP2 and FGF-21 levels(r=0.362,P＜0.05),and both serum IGF2BP2 and FGF-21 levels were positively correlated with fasting insulin(FINS),glycosylated hemoglobin(HbA1c),HOMA-IR,and the β-cell function index(HOMA-β),with correlation coefficients ranging from 0.301 to 0.491(P＜0.05).They were negatively correlated with the insulin sensitivity index(ISI)(r=-0.401 and-0.391,respectively,P＜0.05).Logistic regression analysis showed that IGF2BP2,FGF-21,HbA1c and FINS were risk factors for IR in PCOS patients(OR value ranges from 1.026 to 6.813,P＜0.05).The ROC curve analysis indicated that the combined assessment of serum IGF2BP2 and FGF-21 levels for predicting IR in PCOS patients yielded an area under the curve(AUC)of 0.929,which was significantly higher than that for IGF2BP2 or FGF-21 alone(Z=3.506 and 3.204,respectively,P＜0.05).Conclusion Serum IGF2BP2 and FGF-21 levels are elevated in PCOS patients and are closely associated with insulin resistance.The combination of IGF2BP2 and FGF-21 may have predictive value for assessing the risk of IR in PCOS patients.
polycystic ovary syndromeinsulin like growth factor 2 mRNA binding protein 2insulin resistancefibroblast growth factor 21
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