Prefrontal cortex EAAT2 levels in the pathogenesis of depression in a rat model of chronic unpredictable mild stress
Objective Major depressive disorder rat model was constructed by chronic unpredictable mild stress.The levels of excitatory amino acid transporter 2,glutamate in frontal lobe and the distribution of gut microbiota were detected,and to explore their relationship with the pathogenesis of MDD.Methods Forty SD rats were randomly di-vided into control group,depression group,intervention group(Lac group and Flu group).Control group were fed normally,and others were build molds for 4 weeks.After successful modeling,mild stress continued in the MDD group,Lac and Flu group were supplemented with lactobacillin tablets and fluoxetine for two weeks.The behavior of the rats was evaluated at 4th and 6th week.The content of glutamate,eaat2 mRNA and protein expression were detected,and gut microbiota was analyzed.Results The content of glutamate of depression group was higher than control and intervention group(P＜0.05),and mRNA and protein levels of eaat2 were lower(P＜0.05).The dominant microbiota of all groups was different.Conclusion Gut microbiota,EAAT2 and glutamate level may play an important role in depression-like behavior.
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