Investigation on the role of praziquantel against Schistosoma japonicum induced liver fibrosis and expression change of host miRNA
Objective To investigate the role and mechanism of praziquantel against Schistosoma japonicum egg induced liver fibrosis.Methods BALB/c mice were infected with Schistosoma japonicum as experimental mouse model.In the experimental group,praziquantel at 250 mg/(kg·d) for consecutive 3 days as insecticide treatment and at 600 mg/(kg·d) for 30 days of continuous administration as antifibrotic therapy while in the control group,the mice only given praziquantel insecticide treatment.The extent of liver fibrosis was evaluated by detection on liver hydroxyproline content and other indicators,and by detection on certain micro RNA (microRNA,miRNA) expression levels in liver tissues with real-time quantitative PC R (real-time PCR).Test results of each group were statistically analyzed with t test.Results liver hydroxyproline content of hepatic fibrosis was significantly reduced due to praziquantel treatment.Liver hydroxyproline content in D75 infection group,insecticide group and treatment group were (0.86±0.07),(0.66±0.06) and (0.25±0.05) mg/g liver wet weight respectively,the hydroxyproline content in the treatment group is lower than that in the infection group and insecticide group(t=12.86,P＜0.01).While some significant changes in miRNA expression levels of mouse liver exhibited in the treatment group compared with that in the insecticide group such as the down-regulation of Col I by 62％,miR-223 by 38％,miR-146b by 75％,miR-142-5p by 77％,miR-199a-5p by 40％,miR-34c* by 54％,and miR-195 by 56％.Conclusion Praziquantel may have certain therapeutic effects on schistosomiasis japonica liver fibrosis by modulating the expression of certain host miRNA expression levels.
NETL
NSTL
万方数据
维普
