摘要
目的 评估湖北省赤壁市农村儿童感染贾第虫情况,并初步进行类型分析,为加强贾第虫病的防治提供依据. 方法 从赤壁市进行隐孢子虫调查的儿童粪便样本中,随机抽取20份样本,基于贾第虫磷酸丙糖异构酶(triosephosphate isomerase,tpi)基因,采用巢式PCR检测粪便标本中的贾第虫,对阳性样本的tpi基因片段进行测序,与GenBank中参考序列进行同源性比对分析,确定贾第虫的集聚体类型. 结果 PCR检测结果显示,1份样本为贾第虫阳性,测序后经分析鉴定为集聚体A,与美国威斯康辛州的污水厂污水分离株1503同源性为99％. 结论 该地区农村儿童存在贾第虫感染,应加强贾第虫感染检测和卫生宣教.
Abstract
Objective To evaluate the Giardia infection in chidren from rural areas of Chibi City and analyze the subtype of Giardia based on the triosephosphate isomerase (tpi) gene so as to provide the evidence for the prevention and treatment of Giardia.Methods A total of 20 human fecal specimens were randomly selected from the samples of an investigation on Cryptosporidium from rural children in Chibi City.The DNA of stool samples was extracted and Giardia was detected through nested-PCR of Giarida tpi gene.Results The results of PCR detection identified one case as Giardia infection.By blasting sequence,it belonged to assemblage A,99％ homology with the 1503 isolate of wastewater samples in USA.Conclusion Giardia infection occurred in rural children in the investigated region where the detection of Giardia infection and health education should be strengthened.
基金项目
国家卫生行业科研专项(201302004)
国家卫生行业科研专项(201502021)
国家科技重大专项(ZX201210004-201)
NETL
NSTL
维普
万方数据