河南省新乡市市售牛羊肉弓形虫感染情况调查
Investigation on Toxoplasma gondii infection of commercial beef and mutton in Xinxiang , Henan Province
许培培 1刘峰 2许晶晶 3李浩 1牛含青 1何京 1曹梓轩 1冯龙 1张耀元1
作者信息
- 1. 453000新乡,新乡医学院三全学院
- 2. 453000新乡,华兰生物疫苗有限公司
- 3. 453000新乡,新乡市解放军371医院
- 折叠
摘要
目的 了解新乡市2013年市售牛、羊肉弓形虫感染情况,为弓形虫病的防治提供依据.方法 随机采集新乡市40个农贸市场的牛、羊肌肉组织,其中市区牛肉71份、羊肉77份,郊区牛肉65份、羊肉64份,农村牛肉46份、羊肉45份,用套式PCR检测肉类弓形虫的感染情况. 结果 共检测牛肉182份,其中阳性23份,阳性率为12.64％ (23/182).市区、郊区、农村牛肉弓形虫阳性率依次为12.00％ (9/75)、12.50％ (8/64)和13.95％ (6/43).共检测羊肉191份,阳性22份,阳性率为11.52％ (22/191),市区、郊区、农村羊肉弓形虫阳性率依次为10.96％(8/73)、11.29％ (7/62)和12.50％ (7/56).牛、羊肉弓形虫阴性率在市区、郊区之间差异均无统计学意义(x2=2.153,x2=1.084,P＞0.05).市区、农村之间牛、羊肉阳性率差异有统计学意义(2=6.418,x2=7.005,P＜0.05). 结论 新乡市郊区及农村市售牛、羊肉有弓形虫感染.
Abstract
Objective To investigate the infection of Toxoplasma gondii of commercial beef and mutton in Xinxiang City.Methods Beef and mutton samples were collected randomly at 40 farmer markets,of which,71 beef and 77 mutton samples from urban; 65 beef and 64 mutton samples from suburban; 46 beef and 45 muttonsamples from rural.The nested PCR was used to detect Toxoplasma gondii DNA in the muscle tissue.Results The total Toxoplasma positive rate was 12.64％(23/182) in beef samples.The Toxoplasma positive rates of beef samples from urban,suburban and rural were 12.00％(9/75),12.50％(8/64) and 13.95％(6/43),respectively.The total Toxoplasma positive rate of mutton samples was 11.52％(22/191).The Toxoplasma positive rates of mutton samples from urban,suburban and rural were 10.96％ (8/73),11.29％ (7/62)and 12.50％ (7/56),respectively.There was no statistically significant difference of beef and mutton positive rate between urban and suburban(x2-2.153,x2=1.084,P＞0.05).There was statistically significant difference of beef and mutton positive rate between urban and rural (x2=6.418,x2=7.005,P＜0.05).Conclusion The results showed that the commercial beef and mutton sold in Xinxiang City were infected with Toxoplasma gondii.
关键词
羊肉/牛肉/弓形虫/套式PCR技术Key words
Mutton/Beef/Toxoplasma gondii/Nested PCR引用本文复制引用
出版年
2015
NETL
NSTL
维普
万方数据