Investigation on Toxoplasma gondii infection of commercial beef and mutton in Xinxiang , Henan Province
Objective To investigate the infection of Toxoplasma gondii of commercial beef and mutton in Xinxiang City.Methods Beef and mutton samples were collected randomly at 40 farmer markets,of which,71 beef and 77 mutton samples from urban; 65 beef and 64 mutton samples from suburban; 46 beef and 45 muttonsamples from rural.The nested PCR was used to detect Toxoplasma gondii DNA in the muscle tissue.Results The total Toxoplasma positive rate was 12.64%(23/182) in beef samples.The Toxoplasma positive rates of beef samples from urban,suburban and rural were 12.00%(9/75),12.50%(8/64) and 13.95%(6/43),respectively.The total Toxoplasma positive rate of mutton samples was 11.52%(22/191).The Toxoplasma positive rates of mutton samples from urban,suburban and rural were 10.96% (8/73),11.29% (7/62)and 12.50% (7/56),respectively.There was no statistically significant difference of beef and mutton positive rate between urban and suburban(x2-2.153,x2=1.084,P>0.05).There was statistically significant difference of beef and mutton positive rate between urban and rural (x2=6.418,x2=7.005,P<0.05).Conclusion The results showed that the commercial beef and mutton sold in Xinxiang City were infected with Toxoplasma gondii.