Research on molecular identification and genetic evolution of Paragonimus westermani in China
Paragonimasis is one of the important food borne parasitic diseases in China.The causal agent is Paragonimus westermani,transmitted via freshwater crayfish or crabs,which can parasite in human tissues and organs and various other mammals to cause diseases.Our country is an important P.westermani distribution country,with 25 provinces (cities,districts) reported.In the taxonomic status of P.westermani,it is difficult to fully reveal the molecular diversity only depending on morphological techniques.Recently,the development of molecular biology techniques has great advantages to use a variety of molecular methods for identifying and classifying this trematode.The application of molecular genetic techniques will provide effective tools and methods to illustrate the biology,epidemiology and genetics of P.westermani.However,due to the significant difference in the sensitivity and specificity of molecular detection methods,as well as their time and cost,it needs to use appropriate methods depending on the study occasions.The application of molecular approaches may be better to reveal the epidemiological and evolutionary characteristics of P.westermani,and provide more effective diagnostic methods for the prevention and control of Paragonimus.So we review the molecular tools and methods of the molecular identification and genetic evolution of paragonimus in China.