Effects of Schistosoma japonicum antigens during different development stages on first trimester human placental trophoblast cells
Objective To explore whether Schistosomajaponicum antigens can affect first trimester human trophoblast cells for providing new clues towards clinical parasite control of women during the process of pregnancy in epidemic areas.Methods Human placental tissues were collected by artificial abortion at 6～ 10 weeks of gestation.The trophoblast cells were isolated by proteinase digestion and cultured in vitro.The supernatants were harvested respectively from the cultured cells after having been treated with 25 μg/ml excretory/ secretory antigens from egg(ES),soluble egg antigens (SEA) and soluble worm antigens (SWA) from S.japonicum for 18 h.The level of cytokines in the culture supernatants was measured by ProcartaPlex detection based on enzyme-linked immunosorbent assay (ELISA).Results It is found that ES could significantly promote the secretion of IL-6(155.58±20.07) pg/ml(t=8.472,P＜0.05) and IL-8(12.78±2.35) ng/ml(t=8.006,P＜0.05),while SEA could significantly increase the production of IL-8 (14.18±2.24) ng/ml (t=7.413,P＜ 0.05).SEA could also increase the production of monocyte chemotactic protein 1 (MCP-1)(212.2±14.57) pg/ml (t=15.640,P＜0.05).The levels of TGF-β1 in the presence of ES(78.56±5.71) pg/ml and SWA(66.61±5.55) pg/ml were significantly higher than that of medium control.The secretory tissue inhibitor of metalloproteinases-1(TIMP-1) was increased significantly in the presence of SEA(1 925.93±143.22) pg/ml and SWA (1 749.83±53.91) pg/ml,compared with the medium group.Conclusion This is the first time for exploring the effects of antigens from different development stages of S.japonicum on cytokines secretion of primary-cultured first trimester human trophoblast cells.We found that ES and SEA promoted the secretion of inflammatory cytokines in trophoblast cells.In addition,SWA significantly promoted TGF-β1 and TIMP-1 secretion.These results suggest that the antigens of S.japonicum during the different development stages affect the function of first trimester human trophoblast cells,thus to provide new clues of subsequent pregnancy monitoring in schistosomiasis epidemic areas.
NETL
NSTL
万方数据
维普
