Immunological analysis of the crude and excretory-secretory antigens of muscle larvae from four Trichinella isolates
Objective To identify the components and immunity of the crude and excretory-secretory antigens of muscle larvae of four Trichinella isolates.Methods Sixteen Kunming mice infected with 300 muscle larvae of Trichinella spiralis,respectively,and muscle larvae were collected on the 45th day after infection.The crude muscle larvae antigens(CLA) and the excretory-secretory antigens(ESA) of the muscle larvae were prepared by repeated freeze-thaw and culture procedures,respectively.Polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze the components of the CLA and ESA.Western-blot analysis was performed with sera of normal mice,mice infected with Trichinella,healthy individuals,and patients with trichinosis,schitosomiasis,clonorchiasis,echinococcosis,sparganosis,trichuriasis,paragonimiasis,cysticercosis,hookworm disease.Results By the analysis of SDS-PAGE,the CLA and ESA of the four Trichinella isolates were all presented 8 main bands with Mr 100 000,66 000,49 000,45 000,43 000,30 000,18 000,12 000 (former) and 70 000,16 000(latter) and 66 000,53 000,49 000,43 000,36 000,34 000,30 000,16 000 respectively,and 1 to 4 sub-bands with Mr 21 000～40 000 of the four isolates.Among them,5 immune reaction bands with Mr 95 000,72 000,53 000,49 000 and 43 000 of the CLA,and 4 bands with Mr 53 000,49 000,43 000,40 000 of the ESA were recognized with the sera of patient and mice infected with Trichinella spiralis respectively on Western blot.Conclusion There are some differences in components and immunological reaction of different isolates,Mr 40 000 component of CLA and Mr 95 000,72 000 components of ESA should be the focus of further study.
NETL
NSTL
万方数据
维普
