Evaluation of colloid gold labeled immunochromatographic strip test for rapid detection of canine leishmanial infection in China
Objective To evaluate a colloid gold immunochromatographic strip test(ICT) for detection of canine visceral leishmaniasis.Methods The streptococcal protein G (SPG) was conjugated with colloid gold as detecting reagent,Leishmania donovani antigen and anti-SPG IgY were immobilized on nitrocellulose in proper position,respectively.The dog samples from an endemic area in Longnan city,Wudu district of Gansu province and a non-endemic area of zoonotic visceral leishmaniasis in Zhang Xian County of Gansu province were collected and subjected to detection by the immunochromatographic strip test (ICT),microscopy,PCR,and enzyme-linked immunosorbent assay(ELISA) respectively.The sensitivity of above detection methods was compared.Results It was found that the positive detection rates of dog bone marrow samples from endemic area by microscopy and those of venous blood samples by PCR were 7.62％(8/105) and 48.57％ (51/105) respectively while those of blood samples by ICT and ELISA were 32.38％(34/105) and 33.33％ (35/105) respectively.The results indicated that the microscopic examination detected less positive of the samples than the PCR test,the ICT and the ELISA (x2=43.58,20.12,21.32,P＜0.05).The results also indicated that the PCR test detected more positive of the samples than the microscopic examination,the ICT and the ELISA (x2=43.58,5.71,5.04,P＜0.05).There was no statistical difference in the sensitivity between immunochromatographic strip test and ELISA (x2=0.02,P＞0.05).Conclusion The colloid gold immunochromatographic strip test is proved to be a reliable method for detection of leishmanial specific antibody in dogs.
NETL
NSTL
万方数据
维普
