3种猪带绦虫重组双歧杆菌候选疫苗在家猪体内的mRNA表达
mRNA expression of three kinds of recombinant bifidobacterium vaccines of Taenia solium in domestic pigs
周必英 1刘美辰 1杨凤娇1
作者信息
- 1. 563000遵义,遵义医学院寄生虫学教研室
- 折叠
摘要
目的 检测3种猪带绦虫重组双歧杆菌候选疫苗在家猪体内的表达情况. 方法 将40日龄健康家猪分别用1011克隆形成单位(colony forming units,CFU)的猪带绦虫Bb-TSO45W-4B、Bb-TSOL18和Bb-TSO45W-4B-TSOL18重组候选疫苗对家猪口服灌胃.首次免疫2周后加强免疫1次.于首免后56 d,剖杀家猪,无菌取肝、肺、脾,采用RT-PCR方法检测上述3种候选疫苗在家猪体内的mRNA表达情况. 结果 在家猪肝脏、肺脏和脾脏中均检测到猪带绦虫TSO45W-4B基因的mRNA,其中肝脏和肺脏的相对表达量为3.3,3.3,1肝脏和肺脏的相对表达量高于脾脏;猪带绦虫TSOL18基因在家猪肝脏和脾脏中均有表达,其中肝脏相对表达量为2.6,高于脾脏的1;猪带绦虫TSO45W-4B-TSOL18融合基因在肝脏、肺脏和脾脏中均有表达,相对表达量依次为0.2、2.4、2.1. 结论 3种猪带绦虫重组双歧杆菌候选疫苗可在家猪体内表达,有利于发挥疫苗的免疫效应.
Abstract
Objective To detect the expression of three kinds of recombinant bifidobacterium(Bb) vaccines of Taenia solium in domestic pigs.Methods Healthy domestic pigs of 40 days old were orally administrated with 1011 (colony forming units,CFU) recombinant Bb-TSO45W-4B,Bb-TSOL18 and Bb-TSO45W-4B-TSOL18 vaccines.Two weeks after the first immunization,these domestic pigs were strengthened immunized.56 days after the first immunization,these domestic pigs were killed.The liver,lung and spleen were collected to detect the expression of three kinds of recombinant Bb vaccines of Taenia solium in domestic pigs using reverse transcription-polymerase chain reaction (RT-PCR).Results The TSO45W-4B gene of Taenia solium could be expressed in liver,lung and spleen of domestic pigs.The relative expression was 3.3,3.3,1,respectively.The relative expression in liver and lung was higher than that in spleen;The TSOL18 gene of Taenia solium could be expressed in liver and spleen of domestic pigs.The relative expression was 2.6,1,respectively.The relative expression in liver was higher than that in spleen.The TSO45W-4B-TSOL18 fusion gene of Taenia solium could be expressed in liver,lung and spleen of domestic pigs.The relative expression was 0.2,2.4,2.1,respectively.Conclusion Three kinds of recombinant Bb vaccines of Taenia solium could be expressed in domestic pigs,which would contribute to playing a role in immune effect of vaccines.
关键词
猪带绦虫/重组双歧杆菌疫苗/表达/体内/家猪Key words
Taenia solium/Recombinant bifidobacterium vaccine/Expression/In vivo/Domestic pigs引用本文复制引用
基金项目
国家自然科学基金(81160206)
贵州省科技厅科学技术基金(:[2014] 2178)
贵州省教育厅自然科学研究项目(2010040)
出版年
2015
NETL
NSTL
维普
万方数据