mRNA expression of three kinds of recombinant bifidobacterium vaccines of Taenia solium in domestic pigs
Objective To detect the expression of three kinds of recombinant bifidobacterium(Bb) vaccines of Taenia solium in domestic pigs.Methods Healthy domestic pigs of 40 days old were orally administrated with 1011 (colony forming units,CFU) recombinant Bb-TSO45W-4B,Bb-TSOL18 and Bb-TSO45W-4B-TSOL18 vaccines.Two weeks after the first immunization,these domestic pigs were strengthened immunized.56 days after the first immunization,these domestic pigs were killed.The liver,lung and spleen were collected to detect the expression of three kinds of recombinant Bb vaccines of Taenia solium in domestic pigs using reverse transcription-polymerase chain reaction (RT-PCR).Results The TSO45W-4B gene of Taenia solium could be expressed in liver,lung and spleen of domestic pigs.The relative expression was 3.3,3.3,1,respectively.The relative expression in liver and lung was higher than that in spleen;The TSOL18 gene of Taenia solium could be expressed in liver and spleen of domestic pigs.The relative expression was 2.6,1,respectively.The relative expression in liver was higher than that in spleen.The TSO45W-4B-TSOL18 fusion gene of Taenia solium could be expressed in liver,lung and spleen of domestic pigs.The relative expression was 0.2,2.4,2.1,respectively.Conclusion Three kinds of recombinant Bb vaccines of Taenia solium could be expressed in domestic pigs,which would contribute to playing a role in immune effect of vaccines.
NETL
NSTL
万方数据
维普
