Bioinformatic analysis on animo acid sequence, structure and antigen epitopes of Echinoccocus granulosus protein 14-3-3
Objective To analyze and predict the animo acid sequence,secondary and tertiary structure,and B cell and T cell epitopes of Echinoccocus granulosus protein 14-3-3 with bioinformatics tools for screening epitopes vaccine and diagnostic antigen.Methods The protein secondary structure was analyzed by DNAstar,and B cell and T cell epitopes of Eg14-3-3 were predicted by DNAstar,Biosun software and Propred MHC class-Ⅱ Binding Peptide Prediction Server software,the tertiary structure was predicted with Swiss-model and Rasmol software.Results Eg14-3-3 is a kind of acid protein consisting of 247 amino acids,with Mr 27 940.The secondary structure containing 6 α-helix linked with short β-sheet and coil,the sequence has high variability.14 B cell epitopes and 6 T cell epitopes were predicted.Conclution The study analyzed the sequence,secondary structure and tertiary structure of Eg14-3-3 and 14 B cell epitopes and 6 T cell epitopes predicted.
NETL
NSTL
万方数据
维普
