Serological evaluation on the immunization effect of PfCP-2.9/PfCSP-2 combination malaria vaccine
Objective To analyze and evaluate the specific IgG antibodies level induced by PfCP-2.9/PfCSP-2 combination malaria vaccine. Methods Five New Zealand white rabbits were divided into two groups, three in vaccine immunized group, and two in non-vaccine immunized group. Vaccine immunized group was inoculated PfCP-2.9/PfCSP-2 combination malaria vaccine, non-vaccine immunized group was in-jected with saline. After 3 consecutive immunizations, blood samples were collected and tested by indirect ELISA to detect IgG antibody. Cut-off values were calculated in accordance with the absorbance value (A val-ue) obtained from non-vaccine immunized rabbits. Cut-off value=2.1× average A value of two non-vaccine im-munized rabbits. If absorbance values acquired from immunized rabbit were higher than the cut-off value, it was judged that the vaccine can induce specific IgG antibodies. Results Vaccines can induce specific IgG antibody against PfCP-2.9 and PfCSP-2 recombinant protein, after 4 level of serum dilution (1∶100, 1∶200, 1∶400, 1:800) detection, The A values from two vaccine immunized rabbits were 0.629/0.544, 0.548/0.443, 0.479/0.378, 0.410/0.281, higher than the cut-off value of the corresponding dilution (0.019, 0.017, 0.016, 0.020). Vaccine can induce specific IgG antibodies against PfCSP (per well coated 4 μg/ml polypeptide), af-ter 4 levels of serum dilution (1∶100, 1∶200, 1∶400, 1∶800) detection, The A values from two vaccine immu-nized rabbits were 0.308/0.152, 0.194/0.080, 0.110/0.040, 0.060/0.025, higher than the cut-off value of corre-sponding dilution (0.027, 0.019, 0.018, 0.018);vaccine can induce specific IgG antibody against PfCSP(per well coated with 10 ug/ml polypeptide) , after 4 level of serum dilution(1∶100, 1∶200, 1∶400, 1∶800) detection, A values from two vaccine immunized rabbits were 0.333/0.129, 0.221/0.086, 0.133/0.048,0.075/0.029, higher than the cut-off value of corresponding dilution (0.048, 0.038, 0.036, 0.036). Conclusion PfCP-2.9/PfCSP-2 malaria vaccine can induce high level of IgG antibody. It is a safe, effective and well-tolerated vaccine.
NETL
NSTL
万方数据
维普
