The influence of lyophilization and redissolution to detection on serum antibodies of schistosomasis japonica patients
Objective To discuss the influence of lyophilization and redissolution to detection on serum antibodies of schistosomasis japonica patients. Methods Thirty sera samples of chronic schistosomasis japonica patients were used in this study, Each serum was divided into 2 equal parts, one part of the sera was freeze dried as powder, the sera were redissoluted to the same volume with 0.9% normal saline at room tem-perature, the other part was without treatment and directly saved for further use. Serum samples of the untreat-ed group and lyophilization and redissolution group were all detected by indirect hemagglutination assay (IHA), enzyme linked immunosorbent assays (ELISA) and dipstick dye immunoassay (DDIA) methods for specific antibody of Schistosoma japonicum. The differences of positive rate between the untreated group and lyophilization and redissolution group and among the three detecting methods were statistically analyzed. Re-sults The positive rates of the untreated group detected by IHA, ELISA and DDIA were all 100%. The posi-tive rate of the lyophilization and redissolution group detected by ELISA was 100% (30/30), by DDIA method was 83.3%(25/30) and by IHA method was 80%(24/30). There was no significant difference between the un-treated group and lyophilization and redissolution group and among the three detecting methods. ConclusionThe influence of lyophilization and redissolution to detection on serum antibodies of schistosomasis japonica patients was rather small, so the freeze drying preservation method could be used for long term preservation of sera samples of schistosomasis japonica patients.
NETL
NSTL
万方数据
维普
