Prediction of main characteristics and antigenic epitopes of profilin from Toxoplasma gondii
Objective To predict the main characteristics of profilin from Toxoplasma gondii(TgPRF)and its T/B cell epitopes using bioinformatics.Methods The open reading frame(ORF),physical chemical properties,signal peptide,transmembrane domains,post-translational modification site,sub-cellular localization,secondary structure,surface accessibility,flexibility,hydrophilicity/hydrophobicity and T/B cell epitopes of TgPRF were analyzed and predicted by bioinformatics online analysis programs including ORF finder,Prot-Param,SignalP,TMHMM,MotifScan,WoLF PSORT,PSORT Ⅱ,SOPMA,Bcepred,SYFPEITHI,NetCTL,combining with bioinformatics software(Gene Runner and DNAMAN).Results The open reading frame of TgPRF gene contained 492 bases and the protein was composed of 163 amino acids with listed characters:the molecular mass was 17 555.2,the molecular formula was C764H1160N204O258S7,the value of theoretical isoelectric point was 4.40.There were four zones with surface accessibility ≥ 1.9,five zones with hydrophilicity ≥ 1.9,three zones with flexibility ≥2,ten post-translational modification sites,eight potential B cell epitopes and three combined T/B cell epitopes.Conclusion TgPRF consists of the dominant antigen epitopes,that will provide a theoretical basis for further study on protective immunity of toxoplasma gondii.
NETL
NSTL
万方数据
维普
