Expression and significance of serum miR-503 and miR-186 in patients with vascular dementia caused by ischemic stroke
Objective To explore the expression and clinical significance of serum microRNA(miR)-503 and miR-186 in patients with vascular dementia(VD)caused by ischemic stroke.Methods A total of 126 pa-tients with ischemic stroke admitted to this hospital from December 2020 to December 2022 were selected as the observation group,and were divided into VD group(35 cases)and non-VD group(91 cases)according to the results of follow-up visits 3 months after treatment.Another 61 healthy subjects who underwent physical examination in the same period were selected as the health group.The expression levels of serum miR-503 and miR-186 were detected by quantitative fluorescence PCR(qPCR),and the general data of VD group and non-VD group were compared.Logistic regression model was used to analyze the factors affecting the occurrence of VD in patients with ischemic stroke,and ROC curve was drawn to analyze the efficacy of serum miR-503 and miR-186 in predicting the occurrence of VD after ischemic stroke.Results Serum miR-503 and miR-186 ex-pression levels in non-VD group and VD group were higher than those in healthy group(P＜0.05),and serum miR-503 and miR-186 expression levels in VD group were higher than those in non-VD group(P＜0.05).Pearson analysis showed that there was a significant positive correlation between serum miR-503 and miR-186 expression level in patients with VD caused by ischemic stroke(r=0.462,P＜0.001).There were statistical-ly significant differences in hypertension,NIHSS score,smoking proportion and mRS score between the two groups(P＜0.05).Smoking,hypertension,NIHSS score,mRS score,serum miR-503 expression level and ser-um miR-186 expression level were risk factors for VD in patients with ischemic stroke(P＜0.05).The area under the curve(AUC)of serum miR-503 and miR-186 combined to predict VD in ischemic stroke patients was 0.948,and the sensitivity and specificity were 85.70％and 92.30％,respectively.The prediction efficacy was better than that of serum miR-503 and miR-186 alone(Z=2.784,2.275,P=0.005,0.023).Conclusion Serum miR-503 and miR-186 are highly expressed in patients with VD caused by ischemic stroke,and which have predictive value for the occurrence of VD in patients with ischemic stroke.
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