The clinical value of serum miR-322-5p and IL-1β in the diagnosis of unexplained recurrent abortion and their relationship with Th17/Treg balance
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目的 探讨血清微小RNA(microRNA，miR)-322-5p、白细胞介素(interleukin，IL)-1β诊断不明原因复发性流产(unexplained recurrent spontaneous abortion，URSA)的临床价值及与辅助性T细胞(helper T cell，Th)17/调节性T细胞(regulatory T cells，Treg)平衡的关系。 方法 选取2020年9月至2022年9月海安市人民医院收治的103例URSA患者作为URSA组。另外选取同时期来院产检的120例健康孕妇作对照组。采用实时荧光定量聚合酶链反应检测血清miR-322-5p水平；采用酶联免疫吸附法检测血清IL-1β水平；采用流式细胞仪检测Th17、Treg细胞，计算两者比值。采用Pearson相关分析探讨miR-322-5p、IL-1β与Th17/Treg的关系，采用受试者工作特征(receiver operating characteristic，ROC)曲线评估miR-322-5p、IL-1β对URSA的诊断价值。 结果 与对照组相比，URSA组血清miR-322-5p表达水平明显降低，IL-1β表达水平明显升高，差异有统计学意义[(3.17±1.24)比(1.05±0.43)，(62.14±7.53)pg/L比(81.42±9.16)pg/L，t值分别为16.52，17.25，P值均<0.05]。与对照组相比，URSA组血清Th17水平及Th17/Treg值明显升高，Treg水平明显降低，差异有统计学意义[(1.52±0.42)%比(2.57±0.74)%，(0.28±0.08)比(0.65±0.12)，(6.15±1.52)%比(4.02±1.03)%，t值分别为13.26，27.42，12.04，P值均<0.05]。Pearson相关分析结果显示，URSA患者血清miR-322-5p与Th17、Th17/Treg呈负相关，与Treg呈正相关(r＝－0.41、－0.37、0.50，P值均<0.05)；血清IL-1β与Th17、Th17/Treg呈正相关，与Treg呈负相关(r＝0.37、0.49、－0.42，P值均<0.05)。miR-322-5p、IL-1β预测URSA特异度分别为61.17%、66.02%，敏感度分别为92.50%、83.33%；两者联合预测的特异度为85.44%，敏感度为85.83%。两者联合的诊断价值高于单独检测(Z＝4.102，P＝0.015)。 结论 URSA患者血清miR-322-5p水平明显降低、IL-1β水平明显升高，两者可作为辅助诊断URSA的生物学指标，且与Th17/Treg平衡密切相关。 Objective To explore the clinical value of serum microRNA(miR)-322-5p and interleukin(IL)-1β in diagnosing unexplained recurrent spontaneous abortion(URSA)and its relationship with helper T cell(Th)17/regulatory T cell(Treg)balance. Methods A total of 103 URSA patients admitted to Haian People's Hospital from September 2020 to September 2022 were selected as the URSA group.In addition, 120 healthy pregnant women who came to the hospital during the same period were selected as the control group.Serum miR-322-5p was detected by real-time fluorescence quantitative polymerase chain reaction.Serum IL-1β levels were detected by enzyme-linked immunosorbent assay.The ratio of Th17 and Treg cells was calculated by flow cytometry.Pearson correlation analysis was used to investigate the relationship between miR-322-5p, IL-1β and Th17/Treg.receiver operating characteristic was adopted.ROC curve evaluated the diagnostic value of miR-322-5p and IL-1β in URSA. Results Compared with the control group, the serum miR-322-5p expression level in URSA group was significantly decreased, and IL-1β level was significantly increased[(3.17±1.24) vs (1.05±0.43), (62.14±7.53)pg/L vs (81.42±9.16)pg/L,t=16.52, 17.25, both P values <0.05]. Compared with the control group, the serum Th17 level and Th17/Treg value in URSA group were significantly higher, and the Treg level was significantly lower, the difference was statistically significant[(1.52±0.42)% vs (2.57±0.74)%, (0.28±0.08) vs (0.65±0.12), (6.15±1.52)% vs (4.02±1.03)%, t values were 13.26, 27.42, 12.04, all P values <0.05]. Pearson correlation analysis showed that serum miR-322-5p was negatively correlated with Th17 and Th17/Treg, and positively correlated with Treg ( r values were -0.413, -0.369, 0.502, all P values <0.05). Serum IL-1β was positively correlated with Th17, Th17/Treg, and negatively correlated with Treg ( r values were 0.372, 0.485, -0.416, all P values <0.05). The area under curve(AUC) (95% CI) of miR-322-5p and IL-1β predicted URSA was 0.806 (0.755~0.857) and 0.844 (0.793~0.895), the cut-off values 2.11 and 71.78 pg/L, and the specificity was 61.17% and 66.02%, respectively, the sensitivity 92.50% and 83.33%, respectively. The both combined prediction AUC (95%CI) was 0.921 (0.870~0.972), the specificity 85.44%, and the sensitivity was 85.83%. The diagnostic value of combined detection was higher than that of single detection (Z=4.102, P=0.015). Conclusion The serum miR-322-5p level in Ursa patients was significantly decreased, and IL-1β was increased.Both of them can be used as biological indicators for the diagnosis of URSA, and are closely related to Th17/Treg balance.
Objective To explore the clinical value of serum microRNA(miR)-322-5p and interleukin(IL)-1β in diagnosing unexplained recurrent spontaneous abortion(URSA)and its relationship with helper T cell(Th)17/regulatory T cell(Treg)balance. Methods A total of 103 URSA patients admitted to Haian People's Hospital from September 2020 to September 2022 were selected as the URSA group.In addition, 120 healthy pregnant women who came to the hospital during the same period were selected as the control group.Serum miR-322-5p was detected by real-time fluorescence quantitative polymerase chain reaction.Serum IL-1β levels were detected by enzyme-linked immunosorbent assay.The ratio of Th17 and Treg cells was calculated by flow cytometry.Pearson correlation analysis was used to investigate the relationship between miR-322-5p, IL-1β and Th17/Treg.receiver operating characteristic was adopted.ROC curve evaluated the diagnostic value of miR-322-5p and IL-1β in URSA. Results Compared with the control group, the serum miR-322-5p expression level in URSA group was significantly decreased, and IL-1β level was significantly increased[(3.17±1.24) vs (1.05±0.43), (62.14±7.53)pg/L vs (81.42±9.16)pg/L,t=16.52, 17.25, both P values <0.05]. Compared with the control group, the serum Th17 level and Th17/Treg value in URSA group were significantly higher, and the Treg level was significantly lower, the difference was statistically significant[(1.52±0.42)% vs (2.57±0.74)%, (0.28±0.08) vs (0.65±0.12), (6.15±1.52)% vs (4.02±1.03)%, t values were 13.26, 27.42, 12.04, all P values <0.05]. Pearson correlation analysis showed that serum miR-322-5p was negatively correlated with Th17 and Th17/Treg, and positively correlated with Treg ( r values were -0.413, -0.369, 0.502, all P values <0.05). Serum IL-1β was positively correlated with Th17, Th17/Treg, and negatively correlated with Treg ( r values were 0.372, 0.485, -0.416, all P values <0.05). The area under curve(AUC) (95% CI) of miR-322-5p and IL-1β predicted URSA was 0.806 (0.755~0.857) and 0.844 (0.793~0.895), the cut-off values 2.11 and 71.78 pg/L, and the specificity was 61.17% and 66.02%, respectively, the sensitivity 92.50% and 83.33%, respectively. The both combined prediction AUC (95%CI) was 0.921 (0.870~0.972), the specificity 85.44%, and the sensitivity was 85.83%. The diagnostic value of combined detection was higher than that of single detection (Z=4.102, P=0.015). Conclusion The serum miR-322-5p level in Ursa patients was significantly decreased, and IL-1β was increased.Both of them can be used as biological indicators for the diagnosis of URSA, and are closely related to Th17/Treg balance.
miR-322-5pInterleukin-1βRecurrent abortion of unknown causeHelper T cell 17Regulatory T cells
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