Effect of sevoflurane on mitochondrial Ca2+concentration in HT22 cells
Objective To investigate the effect of sevoflurane on mitochondrial Ca2+concentration and mitochondrial calcium uniporter(MCU)in HT22 cells(mouse hippocampal neuronal cell line).Methods HT22 cells were used for the study.According to the random number table method,HT22 cells were divided into two groups:a control group and a sevoflurane group.Cells in the control group were exposed to the medium containing control solvent,while the sevoflurane group was placed in medium containing 1 mmol/L sevoflurane.All the cells were cultivated an incubator at 37 ℃ and 5％CO2 for 6 h.The cell damage was detected by lactate dehydroge-nase kit.The cell viability was assessed by cell proliferation assay kit.The cytoplasmic-and mitochondrial Ca2+concentrations were as-sessed using the cellular Ca2+probes Calbryte-520 AM and Rhod-2 AM.Mitochondrial localization was performed using the mitochon-drial probe PK Mito Deep Red.The expression of MCU was analyzed by Western blot.Results Compared with the control group,the sevoflurane group showed reduced HT22 cell viability(P＜0.05),aggravated cell damage(P＜0.05),and significantly increased Ca2+concentrations in the cytoplasm and mitochondria(P＜0.05),but no statistical difference was found in MCU expression(P＞0.05).Con-clusion Sevoflurane can cause mitochondrial calcium overload,but does not affect the MCU expression.
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