The effect of A1 astrocytes on cognitive impairment after one-time anesthesia and surgery in infancy mice
Objective To observe the effect of A1 astrocytes on cognitive impairment in infancy mice after single-time anes-thesia and surgery.Methods A total of eight healthy C57BL/6 pregnant mice of SPF grade were gestated for 18 d.The 8-day-old in-fancy mice were randomly divided into two groups(n=27),according to the random number table method:a control group and a single-time anesthesia and surgery group.The infant mice of control group were given 40％oxygen,while the single-time anesthesia and surgery group were given 40％oxygen and 3％sevoflurane anesthesia.After anesthesia,the infant mice of single-time anesthesia and surgery group underwent an exploratory surgery.Three infant mice in each group were killed after anesthesia 1.5 h after establish-ing the single-time anesthesia and surgery model.Brain tissue was taken for frozen sections,and the expression of glial fibrillary acidic protein(GFAP)and complement C3(C3)in the hippocampus was detected using immunofluorescence staining;at 30 d of age,6 mice in each group were sacrificed after anesthesia to the hippocampus,and the content level of postsynaptic density protein(PSD)95 was de-tected by Western blot.The remaining 18 infant mice were subjected to Morris water maze experiments at the age of 30-36 d,recording the escape latency and the platform crossing times.Results Compared to mice in the control group,the escape latency of mice in the Morris water maze navigation experiment was significantly prolonged(all P＜0.05)in the anesthesia surgery group on the day 6 and 7,and the number of platform crossings in the Morris water maze spatial exploration experiment was significantly reduced(P＜0.05)on the day 7.Compared to the mice in control group,the C3/GFAP levels of mice in the hippocampal region were significantly increased in the anesthesia surgery group,indicating that the hippocampal region astrocytes were more polarized towards type A 1(P＜0.05),and the PSD95 expression level was significantly reduced(P＜0.05).Conclusions Single anesthesia surgery in infant mice can cause cogni-tive function impairment.Excessive polarization of astrocytes to type A1 leads to a decrease in the PSD95 level in the hippocampal re-gion.
AstrocyteSevofluraneSingle time surgeryCognitive impairmentInfancy mice
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