Effect of continuous intravenous infusion of lidocaine on early renal injury in septic rats
Objective To investigate the effect of continuous intravenous infusion of lidocaine on early renal injury in septic rats.Methods A total of 160 SPF-grade male SD rats were divided into four groups(n=40)using the random number table method:group sham(group S),group cecal ligation and puncture(group CLP),group lidocaine group(group L),and group normal saline group(group N).In group S,the rats underwent laparotomy followed by immediate suturing,while the other groups underwent cecal ligation and puncture(CLP)to establish a sepsis model.Immediately after modeling,group L group received a loading dose of 10 mg/kg lido-caine,followed by continuous intravenous infusion of lidocaine via the tail vein at 10 mg·kg1·h-1 for 3 h.Group N received the same volume of normal saline.The body weight of rats in all four groups was recorded.Their levels of serum interleukin-6(IL-6),tumor necro-sis factor-α(TNF-α),creatinine(Cr),blood urea nitrogen(BUN),cystatin C(Cys-C),and neutrophil gelatinase-associated lipocalin(NGAL)were measured using enzyme-linked immunosorbent assay(ELISA)immediately after laparotomy(T0),at post-CLP 2 h(T1),at post-CLP 4 h(T2),at post-CLP 8 h(T3),at post-CLP 20 h(T4),and at post-CLP 24 h(T5).The histopathological changes in kidney tissue at T5 were observed by hematoxylin-eosin(H-E)staining,and urine volume was measured from T,to T5.The 72-hour survival rates of the remaining 10 rats in each group were also observed.Results There was no statistical difference in body weight among the four groups(all P＞0.05).At T0,no statistical differences were found in the levels of IL-6,TNF-α,Cr,BUN,Cys-C,and NGAL among the four groups(all P＞0.05).Compared with group S,groups CLP,L,and N showed increases in the levels of IL-6,TNF-α,Cr,BUN,Cys-C,and NGAL and decreases in urine volume from T,to T5(all P＜0.05).Compared with group CLP,group L presented decreases in the lev-els of IL-6,TNF-α,Cr,BUN,Cys-C,and NGAL and increases in urine volume from T1 to T5(all P＜0.05);group N showed reduced Cr levels at T,and T2(all P＜0.05),without statistical difference from T3 to T5(all P＞0.05);there were no statistical differences in the levels of IL-6,TNF-α,BUN,Cys-C,and NGAL from T1to T5(all P＞0.05);urine volume increased at T,(P＜0.05),without statistical differenc-es from T2 to T5(all P＞0.05).Compared with group N,group L showed decreases in the levels of IL-6,TNF-α,Cr,BUN,Cys-C,and NGAL from T1to T5(all P＜0.05),and increases in urine volume from T2 to T5(all P＜0.05),though the difference at T1 was not statistical-ly significant(P＞0.05).In all four groups,the kidney tissue showed intact glomeruli,renal tubules,and interstitial structures,with clear cortical and medullary structures.The morphology of the nephron was normal,without inflammatory cell infiltration observed in the basement membrane or mesangial area.Compared with group S(100％),group CLP(10％),group L(60％)and group N(40％)had a de-creased 72-hour survival rate(all P＜0.05);Compared with the CLP group,group L showed an increased 72-hour survival rate(P＜0.05);there were no statistical differences in 72-hour survival rate among the other groups(all P＞0.05).Conclusions Intravenous infusion of lidocaine can inhibit the expression of IL-6 and TNF-α,relieve inflammation,and alleviate early renal injury in septic rats,providing protective effect on the kidneys.
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