目的 探讨青春期前期和青春期早期雌性小鼠海马齿状回颗粒细胞γ-氨基丁酸（GABA）受体电流的变化。 方法 以雌性小鼠为研究对象，3～4周龄小鼠为青春期前期组（n＝6），5～6周龄小鼠为青春期早期组（n＝6）。采用全细胞膜片钳技术，记录青春期前后海马颗粒细胞GABA受体自发抑制性突触后电流（sIPSC）、微小抑制性突触后电流（mIPSC）和tonic电流，并分析其变化。 结果 青春期前期组和青春期早期组小鼠sIPSC的发放频率分别为（2.22±0.12）、（2.30±0.21）Hz，幅值分别为（19.97±2.01）、（23.80±2.86）pA，两组小鼠sIPSC发放频率和幅值比较差异均没有统计学意义（均P>0.05）；青春期前期组和青春期早期组小鼠sIPSC发放频率和幅值的累积概率比较差异均无统计学意义（均P>0.05）。青春期前期组和青春期早期组小鼠mIPSC的发放频率分别为（0.87±0.08）、（2.15±0.21）Hz，幅值分别为（12.51±0.11）、（29.67±0.19）pA，较青春期前期相比，青春期早期小鼠海马颗粒细胞GABA受体mIPSC发放频率升高（P<0.001），幅值增大（P<0.001）。青春期前期组和青春期早期组小鼠sIPSC发放频率和幅值的累积概率比较差异均有统计学意义（均P<0.001）。青春期前期组和青春期早期组小鼠GABA受体tonic电流分别为（17.40±1.64）、（24.70±2.81）pA，两组小鼠GABA受体tonic电流比较差异具有统计学意义（P<0.05）。 结论 青春期早期雌性小鼠颗粒细胞GABA受体电流较青春期前期有所增强，雌性小鼠进入青春期早期时海马颗粒细胞抑制性活动增强。 Objective To investigate the changes in γ-aminobutyric acid (GABA) receptor currents of hippocampal dentate gyrus granule cells in prepubertal and early pubertal female mice. Methods Female mice were selected as the study objects 3 to 4 -week-old mice were selected as the pre-puberty group (n=6) and 5 to 6 -week-old mice were selected as the puberty group (n=6). The whole-cell patch clamp technique was used to record the spontaneous inhibitory post-synaptic current (sIPSC), mini-inhibitory post-synaptic current (mIPSC), and tonic current of hippocampal granulosa cells in the DG region of pre-pubertal and early pubertal female mice, and their changes were analyzed. Results The frequency of sIPSC in the pre-puberty group and puberty group was (2.22 ± 0.12) Hz and (2.30 ± 0.21) Hz, respectively. The amplitude of sIPSC in the pre-puberty group and the puberty group was (19.97 ± 2.01) pA and (23.80 ± 2.86) pA, respectively. The experimental results showed no significant changes in frequency and amplitude of sIPSC of hippocampal granulosa cells in pre-pubertal and early pubertal mice (all P > 0.05), and no statistical significance in the cumulative frequency and amplitude of sIPSC between two groups (all P > 0.05). The frequency of mIPSC in the pre-puberty group and the puberty group was (0.87 ± 0.08) Hz and (2.15 ± 0.21) Hz, respectively. The amplitude of mIPSC in the pre-puberty group and puberty group was (12.51 ± 0.11) pA and (29.67 ± 0.19) pA, respectively. Compared with the pre-pubertal mice, the frequency and amplitude of mIPSC from hippocampal granulosa cells in early pubertal mice are significantly increased ( P < 0.001). There was also a significant difference in the cumulative frequency and amplitude of sIPSC between the two groups ( P < 0.001). The tonic current of the pre-puberty group and puberty group was (17.40 ± 1.64) pA and (24.70 ± 2.81) pA, respectively, and the tonic current in early pubertal mice was significantly higher than that in pre-pubertal mice ( P < 0.05). Conclusions GABA receptor current is enhanced in early pubertal female mice compared with pre-pubertal females. The inhibitory activity of hippocampal granulosa cells in early adolescent female mice was increased.
Experimental study on GABA receptor currents of hippocampal granulosa cells in pre-pubertal and early pubertal mice
Objective To investigate the changes in γ-aminobutyric acid (GABA) receptor currents of hippocampal dentate gyrus granule cells in prepubertal and early pubertal female mice. Methods Female mice were selected as the study objects 3 to 4 -week-old mice were selected as the pre-puberty group (n=6) and 5 to 6 -week-old mice were selected as the puberty group (n=6). The whole-cell patch clamp technique was used to record the spontaneous inhibitory post-synaptic current (sIPSC), mini-inhibitory post-synaptic current (mIPSC), and tonic current of hippocampal granulosa cells in the DG region of pre-pubertal and early pubertal female mice, and their changes were analyzed. Results The frequency of sIPSC in the pre-puberty group and puberty group was (2.22 ± 0.12) Hz and (2.30 ± 0.21) Hz, respectively. The amplitude of sIPSC in the pre-puberty group and the puberty group was (19.97 ± 2.01) pA and (23.80 ± 2.86) pA, respectively. The experimental results showed no significant changes in frequency and amplitude of sIPSC of hippocampal granulosa cells in pre-pubertal and early pubertal mice (all P > 0.05), and no statistical significance in the cumulative frequency and amplitude of sIPSC between two groups (all P > 0.05). The frequency of mIPSC in the pre-puberty group and the puberty group was (0.87 ± 0.08) Hz and (2.15 ± 0.21) Hz, respectively. The amplitude of mIPSC in the pre-puberty group and puberty group was (12.51 ± 0.11) pA and (29.67 ± 0.19) pA, respectively. Compared with the pre-pubertal mice, the frequency and amplitude of mIPSC from hippocampal granulosa cells in early pubertal mice are significantly increased ( P < 0.001). There was also a significant difference in the cumulative frequency and amplitude of sIPSC between the two groups ( P < 0.001). The tonic current of the pre-puberty group and puberty group was (17.40 ± 1.64) pA and (24.70 ± 2.81) pA, respectively, and the tonic current in early pubertal mice was significantly higher than that in pre-pubertal mice ( P < 0.05). Conclusions GABA receptor current is enhanced in early pubertal female mice compared with pre-pubertal females. The inhibitory activity of hippocampal granulosa cells in early adolescent female mice was increased.
Pubertyγ-Aminobutyric acid receptorInhibitory post-synaptic current
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